Process for obtaining an aqueous extract enriched with small RNAs from a plant material and extracts resulting from the process
A technology of plant material, water extract, applied in the field of cosmetic use
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Embodiment 1
[0066] Embodiment 1: Preparation of the extract of the rice germ (rice) of Poaceae rich in small RNA
[0067] An aqueous extract enriched for low molecular weight RNA (with a maximum length of 150 nucleotides) was obtained from Oryza sativa from the family Poaceae (formerly Poaceae).
[0068] In the first step, 5% powdered rice germ was placed in distilled water, and 10 mM tetrasodium EDTA was added, or 50 g of rice germ powder was placed in 1 kg of distilled water, and 3.8 g tetrasodium EDTA was added. The pH in this step must be alkaline and between 10.5 and 11 for optimal enrichment of extracts with low molecular weight RNA.
[0069] The mixture was stirred at ambient temperature for 2 hours.
[0070] Then with proteases added at 2% each relative to the plant material used (by (which consists of serine endopeptidase) and bromelain) for enzymatic hydrolysis, or 1 g of each enzyme was added to the mixture. Adjust the pH to between 7.5 and 8.
[0071] The mixture was th...
Embodiment 2
[0078] Example 2: Study of the influence of pH and enzymatic hydrolysis in the implementation of the method
[0079] In order to study the influence of pH in the performance of the method according to the invention, extraction tests were carried out at different pH values and on different types of plants.
[0080] These extractions were especially performed on rice germs (as described in Example 1). Comparable results can generally be obtained for all types of plants considered and more particularly described according to the invention.
[0081] Put 5% rice germ powder in water, or add 50 g of rice germ powder and 10 mM tetrasodium EDTA (or 3.8 g) in 1 kg of distilled water. The pH was adjusted to 7 or 11 by adding 1 to 3 mL of concentrated sodium hydroxide; the mixture was then stirred at ambient temperature for 2 hours.
[0082] Sample collection was then performed during the extraction method to observe the enrichment of low-molecular-weight RNA for each extract after...
Embodiment 3
[0094] Example 3: Preparation of extracts of lentils (Lens esculenta) of the family Fabaceae rich in small RNAs
[0095] An aqueous extract rich in low molecular weight RNA (with a maximum length of 150 nucleotides) was obtained from lentils.
[0096] In the first step, the lentils are germinated the day before the extraction method, i.e. 40 g of lentils are covered with distilled water.
[0097] The next day, add distilled water to obtain the 4% equivalent of lentils used in the extraction method.
[0098] The lentils (40 g, a sufficient amount for 1 kg of distilled water) were then crushed and 10 mM tetrasodium EDTA (or 3.8 g) was added. The pH was adjusted to 11 by adding NaOH solution, and the mixture was stirred at ambient temperature for 2 hours.
[0099] Filtration with high porosity is advantageously performed to remove solid debris.
[0100] Protease (relative to the plant material used, 2% bromelain and 2% ) was added to the filtrate for enzymatic hydrolysis, ...
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