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A kind of breeding method of rice variety with high zinc and low cadmium

A technology of high zinc and rice, which is applied in the direction of botany equipment and methods, plant gene improvement, application, etc., can solve the problem that zinc cannot be satisfied, and achieve the effect of increasing the separation ratio

Active Publication Date: 2020-01-14
HANGZHOU ZHONGZE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the average zinc content in ordinary rice on the market is only 10mg / Kg, and it is impossible to meet the demand for zinc with a normal full meal

Method used

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  • A kind of breeding method of rice variety with high zinc and low cadmium
  • A kind of breeding method of rice variety with high zinc and low cadmium
  • A kind of breeding method of rice variety with high zinc and low cadmium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Breeding materials:

[0027] (1) Beibei Zhongze is the parent A. Zhongze Beibei is a japonica rice variety with high zinc content cultivated by Hangzhou Zhongze Biotechnology Co., Ltd. It has applied for the protection of new plant variety rights of the Ministry of Agriculture (application number: 20171224.5). The zinc content in the polished rice of this rice variety is as high as 32.1mg / kg, and the accumulation characteristics of heavy metals are the same as those of ordinary rice.

[0028] (2) Take Jia 33 as parent B. Jia 33 is a new rice variety with low cadmium accumulation bred by Jiaxing Agricultural Science Research Institute, Zhejiang Province. It passed the approval of Jiangsu Variety Approval Committee in January 2007 (Sushendao 200711). This variety has the characteristics of low cadmium accumulation, and the zinc content (12mg / kg) is similar to that of ordinary rice.

[0029] 2. Breeding process of high zinc and low cadmium rice:

[0030] (1) In the ...

Embodiment 2

[0065] Breeding of high zinc and low cadmium rice of embodiment 2

[0066] (1) In the spring of 2012, in Lingshui, Hainan, using Zhongze Beibei as parent A and female parent, crossed with parent B (Jia 33), and obtained 6 seeds of F1 (Zhongze Beibei / Jia 33);

[0067] (2) In the summer and autumn of 2012, Hangzhou, Zhejiang, planted 4 F1 (Zhongzebeibei / Jia 33) generation plants, and backcrossed with the parent A Zhongzebeibei to obtain BC1F1 (Zhongzebeibei / / Zhongzebeibei / Jia 33) on behalf of 31 capsules;

[0068] (3) In the spring of 2013, Lingshui, Hainan, planted 31 backcross BC1F1 (Zhongze Beibei / / Zhongze Beibei / Jia 33) generation plants, and used anther culture to construct a doubled haploid (DH) population;

[0069] (4) In the summer and autumn of 2013, Hangzhou, Zhejiang, planted and harvested DH per plant 0 Substitute seeds, construct 291 DH lines;

[0070] (5) Spring 2014, Lingshui, Hainan, DH 1 generation, using ZnSO 4 Prepare solutions with 10 μmol / L, 20 μmol / L...

Embodiment 3

[0075] Breeding of high zinc and low cadmium rice of embodiment 3

[0076] (1) In the spring of 2012, in Lingshui, Hainan, using Zhongze Beibei as parent A and female parent, crossed with parent B (Jia 33), and obtained 6 seeds of F1 (Zhongze Beibei / Jia 33);

[0077] (2) In the summer and autumn of 2012, Hangzhou, Zhejiang, planted 4 F1 (Zhongzebeibei / Jia 33) generation plants, and backcrossed with the parent A Zhongzebeibei to obtain BC1F1 (Zhongzebeibei / / Zhongzebeibei / Jia 33) on behalf of 31 capsules;

[0078] (3) In the spring of 2013, Lingshui, Hainan, planted 31 backcross BC1F1 (Zhongze Beibei / / Zhongze Beibei / Jia 33) generation plants, and used anther culture to construct a doubled haploid (DH) population;

[0079] (4) In the summer and autumn of 2013, Hangzhou, Zhejiang, planted and harvested DH per plant 0 Substitute seeds, construct 291 DH lines;

[0080] (5) Spring 2014, Lingshui, Hainan, DH 1 generation, using ZnSO 4 Prepare solutions with 10 μmol / L, 20 μmol / L...

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Abstract

The invention discloses a breeding method for a high-zinc and low-cadmium rice variety. The method includes selecting high-zinc rice as a parent A, low-cadmium-accumulating rice as a parent B, and hybridizing the parent A and the parent B to obtain an F1 generation seeds; planting a single cross F1 generation, performing backcross with the parent A, and obtaining BC1F1 seeds; for the BC1F1 generation, using anther culture to construct a doubled haploid population, and harvesting seeds according to a single plant; for a DH1 generation, first adopting a hydroponic method to select a high zinc accumulation type line and then selecting a high zinc content line; for a DH2 generation, adopting different cadmium solution water culture methods, and choosing a high zinc line presenting a low cadmium content; for a DH3 generation, planting a high-zinc and low-cadmium line in ordinary fields; and for a DH4 generation, selecting a high-zinc and low-cadmium rice variety. The bred novel rice varietyis a beneficial combination of micronutrient high zinc and low cadmium, lead and arsenic accumulation, and meets the development demands of green and high quality.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding rice varieties with high zinc and low cadmium. Background technique [0002] Zinc is known as the "flower of life". It is a component of many metalloenzymes or an activator of some enzymes. It participates in the metabolism of more than 80 types of life in the human body. It can improve intelligence, promote growth and development, improve taste, increase appetite, and enhance body immunity. A variety of functions. Zinc mainly exists in seafood and animal offal, and other foods contain very little zinc. WHO and FAO recommend that the daily zinc intake should be above 15mg. Rice is the staple food of our people's three meals a day. At present, the average zinc content in ordinary rice on the market is only 10mg / Kg, and it is impossible to meet the demand for zinc with a normal full meal. Therefore, it is of great significance to carry out high-zinc ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 周晓东沈斌斌周志丹林书达方舟
Owner HANGZHOU ZHONGZE BIOTECH