Injectable high-strength thermosensitive modified chitin-based hydrogel and its preparation method and application

A temperature-sensitive, high-strength technology, applied in the field of biomedical materials and tissue engineering, can solve the problems of weak material strength and achieve good biocompatibility, good biocompatibility, and easy industrial production

Active Publication Date: 2021-08-03
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the temperature-sensitive chitin derivatives are weak in physical gelation at elevated temperatures

Method used

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  • Injectable high-strength thermosensitive modified chitin-based hydrogel and its preparation method and application
  • Injectable high-strength thermosensitive modified chitin-based hydrogel and its preparation method and application
  • Injectable high-strength thermosensitive modified chitin-based hydrogel and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the synthesis of hydrophilic macromolecular cross-linking agent

[0038] 1. Synthesis of benzaldehyde-modified polyethylene glycol (PEG) macromolecular cross-linking agent

[0039] (1) Synthesis of linear bisbenzaldehyde-terminated PEG (DF-PEG)

[0040] Benzaldehyde with linear PEG end group Linear bisbenzaldehyde end group PEG

[0041] Weigh 1.5 gram of PEG 6k and 0.19 gram of 4-carboxybenzaldehyde and place in a 100mL round bottom flask, add 30mL of dried methylene chloride to dissolve, then add 0.15 gram of 4-dimethylaminopyridine (DMAP) and 0.31 gram of dicyclohexylcarbodiimide (DCC), stirred and reacted at room temperature for 24 hours, filtered and collected the filtrate and precipitated in a large amount of ether, and the collected crude product was dissolved, recrystallized and dried in isopropanol to obtain bifunctional benzaldehyde PEG, 79% yield. 1 H NMR (500MHz, CDCl 3 ,δ): 10.1 (hydrogen on the aldehyde functional group), 8.2, 7.9 (hydroge...

Embodiment 2

[0052] Embodiment 2: Preparation of thermosensitive chitin derivative A

[0053] Preparation of thermosensitive hydroxypropyl chitin (HPCH)

[0054] According to our previous research work [Chinese invention patent application publication specification CN201410170871.8], a homogeneous method was used to prepare hydroxypropyl chitin with a low degree of deacetylation in a sodium hydroxide-urea system. Weigh 2 grams of purified chitin (viscosity-average molecular weight is 756,000) and stir and disperse it in 100 grams of aqueous solution containing 11wt% sodium hydroxide and 4wt% urea in advance, freeze overnight at -25°C, take it out at room temperature Under mechanical stirring to thaw it, and then repeat the freezing and thawing twice to obtain the dissolved chitin aqueous solution. Add 11.5 grams of propylene oxide to the obtained chitin solution (100 grams, 2 wt%), and the system is mechanically stirred at 2°C for 2 hours to mix the reactants evenly, then the temperature ...

Embodiment 3

[0060] Example 3: Preparation of thermosensitive ammonia aldehyde in situ reaction modified hydroxypropyl chitin HPCH hydrogel

[0061] The DF-PEG prepared in Example 1 (the molecular weight of PEG is 2000) and the thermosensitive hydroxypropyl chitin HPCH-T1 prepared in Example 2 were respectively dissolved in 0.15M PBS buffer at pH 7.4, and the concentration of A solution of 20 wt% DF-PEG and 3 wt% HPCH-T1. Take 0.047g of DF-PEG solution, 1g of HPCH-T1 solution and 0.14g of PBS buffer solution and mix them uniformly at 2-15°C for half a minute at low temperature (it is liquid for a long time after mixing at low temperature, and has good injectability properties) It was placed in a constant temperature water bath at 37°C and quickly formed a gel, which illustrated its in-situ rapid gelation characteristics in vivo. Because the chemical crosslinking of ammonia aldehyde proceeds slowly in the body, in a constant temperature water bath at 37°C for 6-24 hours, a hydrogel with a ...

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Abstract

The invention discloses an injectable high-strength temperature-sensitive modified chitin-based hydrogel and its preparation method and application. Introducing a cross-linking agent or a photoinitiator to form an injectable hydrogel precursor solution with good fluidity. After being injected into the body, the injectable hydrogel is formed by spontaneous chemical cross-linking or photoirradiation cross-linking under physiological conditions. High strength hydrogel. The precursor material of the hydrogel has abundant sources and is easy to prepare, and what is used is a photoinitiator or a cross-linking agent with good solubility in water and low toxicity. The thermosensitive chitin derivatives are used, which can be physically cross-linked in situ under physiological conditions, and then further chemically cross-linked or photo-crosslinked in situ to prepare injectable high-strength hydrogels without any post-treatment. In addition, this type of hydrogel is biodegradable and can be widely used in fields such as biomedical materials and tissue engineering technology.

Description

technical field [0001] The invention belongs to the field of biomedical materials and tissue engineering, and specifically relates to an injectable intelligent high-strength chitin-based hydrogel product and its preparation method and application. Background technique [0002] Polymer hydrogel is a cross-linked polymer with a three-dimensional network structure that absorbs a large amount of water and is insoluble in water. Because it is close to the extracellular matrix, it has good hydrophilicity, excellent swelling performance and biocompatibility etc., have broad application prospects in the fields of biomedicine and tissue engineering materials. For example, as a medical device, hydrogel can be used for wound dressing, adhesion, sealing and anti-leakage during surgery, hemostasis during surgery, tissue filling, anti-adhesion after surgery, or can evenly wrap cells, proteins, peptides, hormones, etc. Various bioactive substances and drug carriers and tissue engineering ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/52A61L24/04A61L24/08A61L31/04A61L31/06A61L31/14C08B37/08
CPCA61L24/0031A61L24/043A61L27/26A61L27/52A61L31/041A61L31/145A61L2400/04A61L2400/06C08B37/003C08L5/08C08L71/02
Inventor 蒋序林毕波袁蒙黄佳昌
Owner WUHAN UNIV
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