Application of Luo Han Guo extract mogroside V in promoting oocyte maturation in vitro

A technique of Luo Han Guo extract and oocyte, which is applied in the field of in vitro culture of animal oocytes

Active Publication Date: 2020-12-08
GUANGXI UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, these are just some mechanism studies and research status in cells. There is no report on whether mogroside V can promote meiosis, improve oocyte development efficiency, and be used for parthenogenetic activation

Method used

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  • Application of Luo Han Guo extract mogroside V in promoting oocyte maturation in vitro
  • Application of Luo Han Guo extract mogroside V in promoting oocyte maturation in vitro
  • Application of Luo Han Guo extract mogroside V in promoting oocyte maturation in vitro

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Experimental program
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Embodiment Construction

[0023] 1. Obtaining oocytes

[0024] Store the complete sow ovary in a container filled with physiological saline without adding antibiotics. The temperature in the container is 30-34°C, which is the most suitable temperature for transportation. The oocyte cleaning solution used in the process of egg collection and egg retrieval contains double antibodies to ensure the most suitable environment for oocytes from transportation, collection to culture. After all the oocytes are collected in the oocyte cleaning solution, they are ready for the next step of culture. Among them, the composition of the oocyte cleaning solution is shown in Table 1. After the pH was adjusted with NaOH, it was filtered through a 0.22 μM filter and stored at 4° C. for future use.

[0025] Table 1 Components of oocyte washing solution

[0026]

[0027] 2. In vitro maturation of oocytes

[0028] After the GV stage oocytes are obtained from the ovary, they are placed in the oocyte cleaning solution to...

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Abstract

The invention discloses a fructus momordicae extract mogroside V which has a good function of promoting in-vitro maturation of oocyte, and accordingly, an inventor also establishes a relatively complete culture system-a culture solution and a culture method. The culture solution is based on a conventional culture solution, and the fructus momordicae extract mogroside V is added. A research shows that the in-vitro maturation quality of pig ova can be remarkably increased by using the culture solution, an in-vitro maturation rate of the oocyte can be increased from 79.4 percent to 87.4 percent,and a promotion function on ovum developmental potentiality is obtained, so that the in-vitro maturation rate of the oocyte is greatly increased, and the problems that a current maturation efficiencyis low, a culture period is long and large-scale production is not facilitated are solved; by adopting the fructus momordicae extract mogroside V disclosed by the invention, more high-quality oocyte can be provided for somatic cell nuclear transfer, in-vitro fertilization and somatic cell cloning.

Description

technical field [0001] The invention belongs to the field of in vitro culture of animal oocytes, and in particular relates to the application of Mogroside V, an extract from Luo Han Guo, in promoting the in vitro maturation of oocytes. Background technique [0002] In vitro maturation of oocytes is an important technology for in vitro fertilization, cloning and breeding, and the production of transgenic animals. The quality of pig oocytes has a great impact on the development of embryos after in vitro fertilization and activation. In recent decades, the porcine oocyte in vitro culture technology at home and abroad has made great progress, but the in vitro development ability of mature oocytes is still much lower than that of in vivo oocytes, which limits the cloning efficiency and in vitro fertilization efficiency to high-quality oocytes. lack of cells. In recent years, the in vitro maturation technology of porcine oocytes has made great progress, such as the Chinese patent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/12C12N2500/32C12N2500/34C12N2500/50C12N2500/76C12N2500/84C12N2501/11C12N2501/31
Inventor 梁兴伟聂骏宇肖鹏卢克焕
Owner GUANGXI UNIV
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