Construction method and prediction method of woody plant leaf phenotypic characteristics and photosynthetic characteristics prediction model based on DNA methylation level

Abstract

The invention provides a construction method and a prediction method of a woody plant leaf trait and photosynthetic characteristic model based on the DNA methylation level, and belongs to the technical field of biological analysis. The present invention selects important characteristic variables that reflect geographical location differences based on random forests, obtains seven leaf characteristic variables by screening, determines the optimal number of clusters, and uses the improved FCM clustering algorithm to obtain each group of clustered leaf samples; according to the correlation between variables The importance of the enzyme cut combination obtained from the gradient boosting tree is used to obtain the important enzyme cut combination in each group of clustered leaf samples; the DNA methylation level of the enzyme cut combination is used as a regression variable, and the LS is constructed based on the Gaussian radial basis function ‑SVM regression prediction model; input DNA methylation levels of important enzyme cleavage combinations to accurately predict leaf shape factor, leaf area and net photosynthetic rate. The method is used to predict the phenotypic characteristics and photosynthetic characteristics of woody plants, and simultaneously screen woody plant individuals with excellent traits.

Description

technical field [0001] The invention belongs to the field of biological information, and in particular relates to a construction method and a prediction method of a woody plant leaf phenotype characteristic and photosynthetic characteristic prediction model based on DNA methylation level. Background technique [0002] DNA methylation most commonly occurs at the fifth carbon atom of cytosine within a dinucleotide and is an important epigenetic modification. DNA methylation represses the expression of transposable elements, pseudogenes, repetitive sequences and individual genes, and plays a key role in many biological processes such as gene expression, embryonic development, cell proliferation, differentiation and chromosome stability. In plants, DNA methylation occurs at CG, CHG, CHH sites (H stands for bases other than guanine), and in higher plant cells, methylated cytosine can account for up to the total 50% of the number of cytosines. DNA methylation controls plant grow...

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Problems solved by technology

However, the research is still in the qualitative analysis, and there is a lack of quantitative research on DNA methylation analysis

At the same time, the current research on DNA methylation of forest plants relies on the scanning of DNA methylation of the whole genome, the research cost is high, and the large amount of data leads to poor accuracy of results

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