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Biological toxicity detection method for coal chemical industry wastewater

A technology for coal chemical wastewater and biotoxicity, which is applied in the direction of color/spectral characteristic measurement, test water, measuring devices, etc., can solve the problems that water quality biotoxicity detection methods are not suitable for coal chemical wastewater biotoxicity detection, and is easy to popularize and promote , The effect of short detection cycle

Inactive Publication Date: 2018-07-27
HARBIN INST OF TECH
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Problems solved by technology

[0003] In order to solve the problem that the existing water quality biotoxicity detection method is not suitable for the biotoxicity detection of coal chemical industry wastewater, the present invention proposes a biotoxicity detection method of coal chemical industry wastewater

Method used

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  • Biological toxicity detection method for coal chemical industry wastewater
  • Biological toxicity detection method for coal chemical industry wastewater
  • Biological toxicity detection method for coal chemical industry wastewater

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Embodiment

[0046] Embodiment: combine below Figure 1 ~ Figure 4 This embodiment will be described in detail.

[0047] The biotoxicity detection method of the coal chemical industry wastewater described in the present embodiment comprises:

[0048] Step 1, cultivating Tetrahymena to obtain a Tetrahymena solution;

[0049] Step 2, calculate the Tetrahymena density of the Tetrahymena solution, and judge whether the Tetrahymena density is less than 5×10 4 cell / ml, when the judgment result is yes, perform step 1, otherwise, use sterile culture medium to dilute the predetermined volume of Tetrahymena solution 10 times to obtain the Tetrahymena test solution;

[0050] Step 3, preparing a control group, an experimental group, a first blank group and a second blank group;

[0051] The control group is the Tetrahymena test solution, the experimental group is the mixed solution of the Tetrahymena test solution and the water sample to be tested, the first blank group is the sterile culture solut...

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Abstract

The invention provides a biological toxicity detection method for coal chemical industry wastewater and belongs to the technical field of biological toxicity detection, aiming at solving the problemsthat an existing water quality biological toxicity detection method is not applicable to biological toxicity detection of the coal chemical industry wastewater. The biological toxicity detection method adopts tetrahymena as a toxicity testing specie; the tetrahymena commonly exists in the coal chemical industry wastewater and has certain adaptability on biological toxicity substances and the sensitivity of the tetrahymena is not too high, so that the biological toxicity detection device for the coal chemical industry wastewater, provided by the invention, is applicable to detection of the coalchemical industry wastewater. On the other hand, compared with green algae, daphnia magna or zebra fish, the tetrahymena has the advantages of easiness for culturing and short reproductive period. Therefore, compared with an existing water quality biological toxicity detection method based on the green algae, the daphnia magna or the zebra fish, the biological toxicity detection method for the coal chemical industry wastewater has the advantages that a detection period is relatively short and the device is easily popularized and generalized in the field of the biological toxicity detection ofthe coal chemical industry wastewater.

Description

technical field [0001] The invention relates to a biological toxicity detection method of water quality, belonging to the technical field of biological toxicity detection. Background technique [0002] The test species used in the existing water quality biotoxicity detection methods are usually photobacteria, green algae, daphnia magna or zebrafish. Among them, the water quality biotoxicity detection method based on luminescent bacteria is not suitable for the detection of coal chemical wastewater with high toxicity equivalent due to the high sensitivity of luminescent bacteria to biological toxic substances. However, when the water quality biotoxicity detection methods based on green algae, Daphnia magna or zebrafish are used to detect the biotoxicity of coal chemical wastewater, there is a problem that the detection period is too long, which seriously limits the use of these three water quality biotoxicity detection methods. Application in the field of biotoxicity detecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/18
CPCG01N21/31G01N33/1866
Inventor 韩洪军郑梦启徐春艳张正文
Owner HARBIN INST OF TECH
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