Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of serum inflammatory factors

A technology of inflammatory factors and serum, applied in the field of medicine, can solve problems such as complicated diagnosis basis

Active Publication Date: 2018-07-27
徐昉
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] It can be seen that the basis for the diagnosis of sepsis is complicated, and early diagnosis of sepsis means early treatment, so the diagnosis of sepsis is the focus and difficulty of current research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of serum inflammatory factors
  • Application of serum inflammatory factors
  • Application of serum inflammatory factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The human serum used in the experiment was the serum of patients with sepsis in the intensive care unit of the First Affiliated Hospital of Chongqing Medical University and the serum of normal physical examination subjects. The experimental animals used in the animal experiments were C57bl / 6 mice, and the method of inducing sepsis was cecal knot puncture.

[0054] The specific method is to collect the serum of clinical patients, use the human IL-38 Elisa kit to detect the serum IL-38 level of the patient, and compare it with the standard level to determine whether there is an increase (the standard level is the normal human serum IL-38 concentration measured, different manufacturers Elisa The normal value measured by the kit is different), if there is an increase, it is positive, and the combination of sepsis diagnosis indicators can improve the early diagnosis rate of sepsis.

[0055] The experimental method is to put the collected blood of patients or volunteers into ...

Embodiment 2

[0069] The mice were subjected to cecal puncture (CLP) to induce sepsis in the mice, and the mice were killed 6 hours, 12 hours, and 24 hours after the operation, and the blood of the mice was collected, and the blood of the mice was placed at 4 degrees Celsius and 3000 rpm. Centrifuge in a centrifuge for 10 minutes, collect the mouse serum, and use enzyme-linked immunosorbent assay (ELISA) to measure the concentration of IL-38 in the mouse serum. The raw data are shown in the following table.

[0070] Table 5 Raw data of IL-38 significantly increased in serum of sepsis model mice

[0071]

[0072]

[0073] N, 6H, 12H, 24H respectively correspond to normal mice and 6 hours, 12 hours and 24 hours after the treatment of sepsis, it is the first time to prove that IL-38 is elevated in the serum of septic mice, and the statistical detection is T-test, meaningful. The original light transmittance was converted to IL-38 concentration through the standard curve. Then the four ...

Embodiment 3

[0094] See Example 2 for the method, and the assay index is interleukin-17A. The results are shown in Table 11.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of medicine, particularly to application of serum inflammatory factors. Experiments show that the concentration of interleukin-38 of sepsis patients is significant in increase compared with that of healthy people. The application of serum inflammatory factors adds a reliable index for diagnosis of sepsis and provides important help for improving the promptness and accuracy of diagnosis of sepsis. The application of serum inflammatory factors aims at improve the early diagnosis rate of sepsis to achieve prompt diagnosis and treatment of sepsis and reduce the death rate of sepsis. Besides, experiments also show that the interleukin-38 achieves therapeutic effects on sepsis.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of serum inflammatory factors. Background technique [0002] Sepsis refers to the systemic inflammatory response syndrome (SIRS) caused by infection, and the presence of bacteria or highly suspicious infection foci is clinically confirmed. Although sepsis is caused by infection, once it occurs, its occurrence and development follow its own pathological process and rules, so in essence, sepsis is the body's response to infectious factors. [0003] Sepsis can be caused by infection at any site, and it is common clinically in pneumonia, peritonitis, cholangitis, urinary system infection, cellulitis, meningitis, abscess, etc. The pathogenic microorganisms include bacteria, fungi, viruses, and parasites, etc., but not all patients with sepsis have positive blood culture results for pathogenic microorganisms that cause infection, and only about 45% of patients with septic shock...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68A61K38/20A61P31/04A61P31/10A61P31/12A61P33/00
CPCA61K38/2086G01N33/6863G01N33/6866G01N33/6869G01N2333/521G01N2333/54G01N2333/5406G01N2333/5412G01N2333/5428G01N2333/5437G01N2333/55G01N2333/57G01N2333/7151
Inventor 徐昉柴雨森林时辉王川江雷铭
Owner 徐昉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com