Specimen preserving fluid

A technology for preserving liquid and specimens, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of yellowing, specimen hardening, mildew, etc., and achieve lasting effects, lightening burden, and no irritating odor. Effect

Inactive Publication Date: 2018-08-24
黄名义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the specimens preserved in formaldehyde solution for a long time will have mildew, yellowing, and hardening of the specimens.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A specimen preservation solution is prepared by mixing the following raw materials in proportions by weight:

[0022] Potassium sorbate 3 parts, industrial ethanol 5 parts, laurocapram 1 part, edetate disodium zinc salt 1 part, protein cross-linking agent 0.8 part, penetrant 1 part, glycerin 1 part, glutaraldehyde 1 part , 7 parts of sodium chloride, 8 parts of citric acid, and 80 parts of deionized water.

[0023] The protein cross-linking agent is a combination of malondialdehyde and glyoxal.

[0024] The penetrant is a combination of sodium chloride and sodium nitrate.

[0025] The mass fraction of glutaraldehyde is 48%.

Embodiment 2

[0027] A specimen preservation solution is prepared by mixing the following raw materials in proportions by weight:

[0028] 4 parts of potassium sorbate, 6 parts of industrial ethanol, 2 parts of laurocapram, 2 parts of disodium zinc salt of edetate, 1 part of protein cross-linking agent, 1.5 parts of penetrant, 2 parts of glycerin, 1.5 parts of glutaraldehyde parts, 8 parts of sodium chloride, 10 parts of citric acid, and 90 parts of deionized water.

[0029] The protein cross-linking agent is a combination of glyoxal and succinaldehyde.

[0030] The penetrating agent is a combination of sodium nitrate and potassium chloride.

[0031] The mass fraction of glutaraldehyde is 50%.

Embodiment 3

[0033] A specimen preservation solution is prepared by mixing the following raw materials in proportions by weight:

[0034] Potassium sorbate 5 parts, industrial ethanol 7 parts, laurocapram 3 parts, edetate disodium zinc salt 3 parts, protein cross-linking agent 1.2 parts, penetrant 2 parts, glycerin 3 parts, glutaraldehyde 2 parts parts, 9 parts of sodium chloride, 12 parts of citric acid, and 100 parts of deionized water.

[0035] The protein cross-linking agent is a combination of malondialdehyde and succinaldehyde.

[0036] The penetrating agent is a combination of sodium chloride and potassium chloride.

[0037] The mass fraction of glutaraldehyde is 52%.

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PUM

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Abstract

The invention discloses a specimen preserving fluid. The specimen preserving fluid is prepared from raw materials in parts by weight as follows: 3-5 parts of potassium sorbate, 5-7 parts of industrialethanol, 1-3 parts of laurocapram, 1-3 parts of disodium zinc ethylenediaminetetraacetate tetrahydrate, 0.8-1.2 parts of a protein crosslinking agent, 1-2 parts of a penetrant, 1-3 parts of glycerin,1-2 parts of glutaraldehyde, 7-9 parts of sodium chloride, 8-12 parts of citric acid and 80-100 parts of deionized water. The specimen preserving fluid is a colorless and transparent liquid, has no pungent smell, has excellent sterilization and anti-mold property, can effectively keep the tissue form and color and luster of an original biological specimen, contains no volatile substances, has lasting effects, can effectively reduce the cost, does not contain toxic or harmful substances, is green and environmentally friendly and can relieve the burden on the environment.

Description

technical field [0001] The invention relates to the technical field of pathological tissue preservation, in particular to a specimen preservation solution. Background technique [0002] From the operating room to the pathology department, laboratory surgery, tissue biopsy, histopathology department, gastroscopy department, etc., it is necessary to use specimen preservation solution for fixing, storing and retaining samples of tissues or cells. The existing preservation solution is mainly Fu formalin preservation solution. Formalin specimen preservation solution has a good fixation effect, which is due to the large amount of formaldehyde combined with the functional groups of the specimen protein, and after long-term storage, it further polymerizes to form paraformaldehyde. This process makes it form a moderate cross-link between protein peptide chains, which can not only kill bacteria, but also maintain the tissue shape of the specimen. However, formaldehyde and benzene ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00A01N1/02
CPCA01N1/00A01N1/021A01N1/0215
Inventor 黄名义
Owner 黄名义
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