Preparation method of radix ginseng rubra extract

Abstract

The invention relates to a preparation method of radix ginseng rubra extract. The preparation method is characterized by comprising the following steps: A, under the aseptic condition, taking an appropriate amount of liquid culture medium, and preparing a fungal suspension with the strain concentration being 1.0x10<5>-1.0x10<6> CFU / mL; B, taking 0.3-0.5 mL of the fungal suspension, carrying out scribing on the surface of a PDA solid culture medium, carrying out culturing for 3-4 days at 20-25 DEG C, and carrying out expanding culture in a fermentation shake flask, thus obtaining the cordycepssinensis hypha fermentation seed culture solution; C, carrying out washing, drying, sterilizing and grinding on radix ginseng rubra, and carrying out wall breaking by adopting a high-pressure microjethomogenizer, thus obtaining wall-broken homogenate of radix ginseng rubra; D, loading the cordyceps sinensis hypha fermentation seed culture solution and the wall-broken homogenate of radix ginseng rubra into a fermentation tank at a certain mass ratio, and carrying out fermenting; E, after the fermentation is completed, carrying out decoloring, carrying out adsorbing, carrying out filtering, andcarrying out drying, thus obtaining crude extract; and F, carrying out hot water extraction on the crude extract, thus obtaining the radix ginseng rubra extract. The preparation method has the advantages that the process is simple, the yield of radix ginseng rubra prosapogenin in the radix ginseng rubra extract is high, the content of cordycepin is high, and meanwhile, the purpose of no pollution and environment pollution is achieved.

Description

technical field [0001] The invention relates to the technical field of natural product biological fermentation, in particular to a preparation method of red ginseng extract. Background technique [0002] Red ginseng (Radix Ginseng Rubra) is the most common processed product of ginseng. It is made through infiltration, cleaning, sorting, steaming, drying, drying and other processes. The active ingredients mainly include saponins, polysaccharides, fat-soluble and volatile substances, etc. . Among them, red ginseng saponins have been widely studied and applied because of their functions of delaying aging, anti-fatigue, anti-oxidation, enhancing immunity, and inhibiting tumor cell growth. However, the main saponins Rb1, Rb2, Rc, Rd, Re, Rg1, Due to the low absorption rate of the human body, the pharmacological effects of Ro, etc. cannot be fully exerted, while the secondary saponins Rg3, Rg5, Rh1, Rh4, Rk1, etc., have high absorption rates in the human intestine, which play an ...

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for preparing secondary saponins mainly include heat treatment, acid hydrolysis, enzymatic conversion and organic synthesis, but red ginseng may generate carcinogens such as 5-hydroxymethylfurfural (5-HMF) during heat treatment; In the process of preparing saponin by acid hydrolysis method and enzyme conversion method, impurities or bacteria will inevitably be introduced, and the products obtained are not suitable for use as food or cosmetic raw materials; the organic synthesis method route and process are more complicated, the output is very low, and the cost is high High and not conducive to environmental protection

The application and development of secondary saponins are greatly restricted due to factors such as low yield and high price

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