Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Two chain peptide compounds preparation method and application thereof

A compound and peptide technology, applied in the field of microbial engineering, can solve the problem of decreased sensitivity to artemisinin, and achieve the effects of short cycle, guaranteed source, and simple process

Inactive Publication Date: 2018-09-04
SHENYANG AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the combination therapy based on artemisinin has been outstanding in clinical application, the sensitivity monitoring of artemisinin drugs in recent years has confirmed that the sensitivity of Plasmodium falciparum to artemisinin has decreased significantly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Two chain peptide compounds preparation method and application thereof
  • Two chain peptide compounds preparation method and application thereof
  • Two chain peptide compounds preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Isolation and purification of entomopathogenic nematode symbiotic bacteria Xenorhabdus budapestensis.

[0024] Put the fifth instar of the wax moth into the soil sample, then turn the soil sample upside down repeatedly, so that the wax moth is evenly distributed in the soil sample, store the soil sample in a constant temperature environment of 25 ℃, and put the Take it out, use 75% alcohol and 0.01% sodium hypochlorite to disinfect the epidermis of the wax moth corpse, then rinse it with sterile water for 2-3 times, dry the water on the wax moth corpse with sterile filter paper, and cut off the gastropods At the top, the hemolymph was dropped onto the NBTA medium, three-section line was drawn, and cultured at 28°C for 24-48h. After the bacterium grows, pick a single colony for purification and culture to obtain the entomopathogenic nematode symbiotic bacteria. Through 16S rRNA gene evolution analysis and homologous gene sequence comparison, it is identified as the pa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a two chain peptide compounds preparation method and an application thereof. The two chain peptide compounds relate to chain peptide compounds Rhabdopeptide J and Changshamycin D, which are obtained by separating insect pathogenic nematode symbiont bacteria Xenorhabdus budapestensis liquid fermentation object, the compounds have inhibition activity for plasmodium, and canbe used for preparing an anti-plasmodium drug. the compounds can be produced by using microbe for liquid fermentation, and has the advantages that the technology is simple, the period is short, the cost is low, the source is guaranteed, a biology method is used for synthesis, and no pollution is generated on environment.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and specifically relates to two chain peptide compounds (Rhabdopeptide J and Changshamycin D) extracted from a liquid fermented product of entomopathogenic nematode symbiotic bacteria Xenorhabdusbudapestensis and its preparation method and application. Background technique [0002] Entomopathogenic nematode symbionts are a type of Enterobacteriaceae bacteria that parasitize in the intestinal tract of entomopathogenic nematodes, and have dual characteristics of being pathogenic to host insects and symbiotic with host nematodes. It includes Xenorhabdus symbiotic with Steinernema and Photorhabdus symbiotic with Heterorhabditis. Entomopathogenic nematode symbiotic bacteria can produce a variety of metabolites with antibacterial, insecticidal, nematicidal, anti-ulcer, anti-tumor and anti-viral activities. They have great development potential and application prospects in the fields of ag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/06C07K1/16C12P21/02A61P33/06C12R1/01
CPCA61P33/06C07K1/16C07K7/06C12P21/02Y02A50/30
Inventor 于志国陈启军毕于慧王建花
Owner SHENYANG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com