A kind of fluid hemostatic glue and its preparation method and application
A fluid and gelatin technology, used in applications, pharmaceutical formulations, surgery, etc., can solve problems affecting wound healing, tissue irritation, etc., and achieve good hemostasis, easy operation, and increased adhesion.
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Embodiment 1
[0035] A fluid hemostatic glue, comprising a hemostatic effective component and a fluid matrix, the hemostatic effective component includes component A, component B and component C, wherein component A is 0.5g gelatin, and component B is 0.5g α-glycerin Sodium Phosphate, Component C is 0.5g polylysine, the fluid base consists of 10g saline and 0.5mg glycerin. Wherein the jelly strength of gelatin is 200 Bloom / g, and the source of gelatin is pig source.
[0036] The preparation method of the fluid hemostatic gel is as follows: Weigh 0.5g gelatin, 0.5g α-sodium glycerophosphate and 0.5g polylysine in a container, put in an ice bath, keep the temperature at 2°C, add 10g normal saline, and weigh another Add 0.5mg of glycerin into a container, stir to make it evenly mixed, take it out, put it into a container, and irradiate to obtain a fluid hemostatic gel product, which is stored at low temperature for future use.
Embodiment 2
[0038] A fluid hemostatic glue, comprising a hemostatic effective component and a fluid matrix, the hemostatic effective component includes component A, component B and component C, wherein component A is 0.5g gelatin, and component B is 0.5g α-glycerin Sodium Phosphate, Component C is 0.5g polylysine, the fluid base consists of 10g saline and 0.5mg glycerin. Wherein the jelly strength of gelatin is 200 Bloom / g, and the source of gelatin is pig source.
[0039]Wherein the gelatin of component A is modified gelatin, and the preparation method of the modified gelatin is: weigh 1g of gelatin, dissolve it in 20ml of deionized water, add 0.0125g of EDC and NHS, stir and react at 30°C for 3h, Add ethanol to soak and wash, repeat three times, pre-freeze at -80°C, and freeze-dry using a low-temperature and low-pressure freezer to obtain modified gelatin.
[0040] The preparation method of the fluid hemostatic gel is as follows: Weigh 0.5g of the above-mentioned modified gelatin, 0.5g...
Embodiment 3
[0042] A fluid hemostatic gel, comprising a hemostatic effective component and a fluid matrix, the hemostatic effective component includes component A, component B and component C, wherein component A is 1 g of gelatin, and component B is 0.6 g of β-glycerophosphate Sodium, Component C was 0.6 g polyethyleneimine, and the fluid base consisted of 11 g saline and 2.2 mg sodium lauryl sulfate. Wherein the jelly strength of gelatin is 300 Bloom / g, and the source of gelatin is pig source.
[0043] Wherein the component A gelatin is modified gelatin, the preparation method of the modified gelatin is: weigh 2g of gelatin, dissolve it in 20ml of deionized water, add 0.0250g of EDC and NHS, and stir and react at 25°C for 2.5h , add ethanol to soak and wash, repeat three times, pre-freeze at -80°C, and freeze-dry using a low-temperature and low-pressure freezer to obtain modified gelatin.
[0044] The preparation method of the fluid hemostatic gel is as follows: Weigh 1g of the above-m...
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