Lensless holographic microscopic phase recovery method with multi-constraint information and device thereof

Abstract

The invention discloses a lensless holographic microscopic phase recovery method with multi-constraint information and a device thereof. The method comprises the following steps: step S1 of turning off a light source, and collecting a dark field image by using a lensless holographic microscopic device; step S2 of turning on the light source to collect a bright field image under uniform illumination of the light source; step S3 of placing a sample above an image sensor to ensure that the distance between the sample and the image sensor is much smaller than that between the sample and the lightsource; turning on the light source to collect a holographic image sequence; step S4 of performing flat field correction on any holographic image needing to be calculated; step S5 of performing an autofocus algorithm on the image subjected to flat field correction, and finding the position of the sample in space; and step S6 of using a phase recovery algorithm with multi-constraint information forthe autofocus image having undergone automatic focusing, to reconstruct the accurate amplitude and phase information of the sample. According to the method and device, the accurate phase informationcan be recovered from the captured holographic image without increasing the complexity of a system and the number of images acquired.

Description

Technical field [0001] The invention belongs to the field of lensless microscopy, and particularly relates to a lensless holographic microscopy phase recovery method and device with multiple constraint information. Background technique [0002] The optical inspection of pathological sections is the gold standard in the field of disease diagnosis. In order to obtain high resolution in traditional optical microscopes, objective lenses with magnification must be used. In order to obtain images with high resolution, wide field of view and large depth of field, it is necessary to rely on mechanical scanning, image stitching and focus adjustment to expand the field of view and depth of field, which not only complicates the imaging process, but also significantly increases the overall cost of the system. Even so, the depth of field and time resolution will still be affected. [0003] Lensless holographic microscope has become a new imaging technology in recent years. Compared with the ...

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Problems solved by technology

[0004] In previous studies, using the error-reduction algorithm (Mudanyali, Onur, et al."Compact, light-weight and cost-effective microscope based on lensless incoherentholography for telemedicine applications."Lab on a Chip 10.11(2010):1417-1428 .) Phase recovery of images captured by lensless holographic microscopy has a large number of iterations, slow convergence speed, is easily affected by twin image noise, and cannot obtain high-quality reconstruction results

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