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Method for carrying out visualized determination on melamine in dairy products based on interface movement distance signals

A technology for melamine and dairy products, applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of high detection cost, expensive instruments, complex pretreatment and professional technicians, etc., and achieves low cost and operation. simple effect

Active Publication Date: 2018-09-11
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Existing melamine detection methods include mass spectrometry, high performance liquid chromatography, enzyme-linked immunosorbent assay and optical methods, etc. These techniques can effectively detect the concentration of melamine, but they require expensive instruments, complicated pretreatments and professional techniques personnel, and the detection cost is high, it is difficult to apply for on-site rapid detection of melamine in dairy products

Method used

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  • Method for carrying out visualized determination on melamine in dairy products based on interface movement distance signals
  • Method for carrying out visualized determination on melamine in dairy products based on interface movement distance signals
  • Method for carrying out visualized determination on melamine in dairy products based on interface movement distance signals

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Embodiment 1

[0029] In this embodiment, the detection of melamine in milk is taken as an example. Firstly, the ET-NB test is carried out on the milk samples added with various concentrations of melamine standard products, and the displacement distances of the neutralization interfaces corresponding to each concentration of melamine are obtained, so as to construct the concentration of melamine. and the standard curve of the displacement distance of NB ( image 3 B. image 3 D shown). After constructing the standard curve, the same method can be used to measure the milk with unknown concentration of melamine. After measuring the displacement distance of the neutralization interface of milk containing unknown concentration of melamine, the concentration value can be calculated according to the displacement distance and the standard curve.

[0030] Such as figure 2 As shown, it is the ET-NB portable direct-reading chip used in this embodiment, which includes: a sealing layer and a channel ...

Embodiment 2

[0044] In this embodiment, the milk in Example 1 is replaced by infant formula milk powder, and five concentration points are selected, which are 0.1, 1, 8, 20, and 40 μM, and infant formula milk powder samples containing known concentrations of melamine are prepared for ET-NB Test, the standard curve of the displacement distance of a melamine concentration and NB interface can be obtained similarly, y=a lg (C MEL )+b, in this embodiment, a is -3.7445, and b is 20.4862, such as image 3 D shows. The content of melamine in the infant formula milk powder sample can be obtained by using the same method in the above-mentioned examples.

[0045] The gel is also an agarose gel, and its concentration and components are consistent with those in the milk ET-NB test.

[0046] Both the background electrolyte solution and the cathode buffer are alkaline buffers of 30mMKCl and 30mMNaOH.

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Abstract

The invention discloses a method for carrying out visualized determination on melamine in dairy products based on interface movement distance signals. The method comprises the following steps: carrying out ET (Electrophoretic Titration)-NB (Neutralization Boundary) sensor test on dairy product samples in a portable direct-read chip to acquire different displacement distances of corresponding NB ofthe melamine with different concentration under known conditions and building a linear relation between the melamine with the known concentration and the NB displacement distance; then carrying out the same ET-NB sensor test on to-be-detected objects under the same conditions to acquire the concentration of the melamine in the to-be-detected objects from the portable direct-read chip according tothe linear relation in a visualized manner. According to the method disclosed by the invention, based on a model of the ET sensor of the NB, the visualized NB is produced in a chip channel through the ET, so that the visualized ET of the melamine in the diary products on the site can be realized.

Description

technical field [0001] The invention relates to a technology in the field of food safety, in particular to a visual method for measuring melamine in dairy products based on an interface moving distance signal. Background technique [0002] Existing melamine detection methods include mass spectrometry, high performance liquid chromatography, enzyme-linked immunosorbent assay and optical methods, etc. These techniques can effectively detect the concentration of melamine, but they require expensive instruments, complicated pretreatments and professional techniques Personnel, and detection costs are high, it is difficult to apply to the rapid detection of melamine in dairy products on-site. Contents of the invention [0003] The present invention aims at the above-mentioned deficiency that existing detection technology exists, proposes a kind of visual method based on interface moving distance signal to measure melamine in dairy products, based on the model of the electrophore...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 曹成喜李文林樊柳荫张强孔昊
Owner SHANGHAI JIAO TONG UNIV