Rapid amplification kit and amplification method for UGT1A1 gene

Abstract

The invention discloses a rapid amplification kit and amplification method for UGT1A1 gene, wherein the rapid amplification kit for UGT1A1 is composed of UGT1A1*6 amplification unit and a UGT1A1*28 amplification unit, wherein the UGT1A1*6 amplification unit includes a specific primer to amplify UGT1A1*6 shown as in sequence table SEQ ID NO: 1; the UGT1A1*28 amplification unit includes a specific primer to amplify UGT1A1*28 shown as in sequence table SEQ ID NO: 2. A multiple PCR (polymerase chain reaction) method is used herein, amplifying is performed on gene polymorphic sites of UGT1A1*6 andUGT1A1*28, an amplifying system is stable and efficient, UGT1A1*6 and UGT1A1*28 sites to be detected can be amplified quickly and accurately at the same time, and amplification primers herein have high specificity, the products are complete, and good basis is laid for subsequent genetic sequencing.

Description

technical field [0001] The invention relates to a rapid amplification kit and amplification method of UGT1A1 gene, belonging to the field of biological detection. Background technique [0002] Irinotecan (CPT-11) is a semi-synthetic water-soluble camptothecin derivative, which mainly acts on the S phase of the cell cycle, inhibits the repair of DNA single-strand breaks, interferes with DNA replication and transcription, and exerts cytotoxic effects It is effective for metastatic colorectal cancer, lung cancer and other tumors, and is currently widely used in the treatment of gastrointestinal cancer and small cell lung cancer. Irinotecan is hydrolyzed into 7-ethyl-10-hydroxycamptothecin (7-ethyl-10-hydroxy-camptothecin, SN-38) by carboxylesterases (CE) in human tissues, SN-38 As inhibitors of DNA topoisomerase Ⅰ (Topo Ⅰ), CPT-11 and SN-38 act on the S phase of the cell cycle. By inhibiting Topo Ⅰ, DNA single-strand damage is induced and DNA replication is blocked to produce ...

AI Technical Summary

Problems solved by technology

However, the existing UGT1A1 detection is based on conventional genetic detection methods. The detection cycle is long and the cost is high, which is not conducive to the rapid identification before medication. Therefore, a rapid amplification diagnostic reagent for UGT1A1 with simple operation and low price is developed. box is necessary

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