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Lead-binding protein gene, protein comprising same and application thereof

A technology for binding proteins and amino acids, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of heavy metal adsorption rate, etc., and achieve the effect of improving adsorption and absorption rate

Active Publication Date: 2020-06-05
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the key core issue of the microbial adsorption method is the heavy metal adsorption rate.

Method used

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  • Lead-binding protein gene, protein comprising same and application thereof
  • Lead-binding protein gene, protein comprising same and application thereof
  • Lead-binding protein gene, protein comprising same and application thereof

Examples

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Embodiment

[0035] (1) Artificial synthesis, plasmid construction and protein expression of the lead-binding protein Flagellin gene derived from sewage bacteria

[0036] Serratia spp., which is tolerant to high concentrations of lead, was isolated from lead-contaminated soil. Metalloprotein analysis was performed on this bacterium, and a lead-binding protein was found. Further analysis and identification of the protein determined its gene sequence , due to the low expression level of this protein in Serratia, on the premise of not changing the amino acid sequence encoded by the gene, we redesigned and directly synthesized it based on the gene sequence of the lead-binding protein obtained from Serratia. The target coding gene is amplified by PCR technology using synthetic cDNA as a template, the amplified fragment is ligated with the pUC57 vector digestion product, and reacted overnight at 16°C to obtain the transformed plasmid of the target gene. Escherichia coli in the logarithmic growth...

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Abstract

The invention discloses a lead binding protein gene as well as a protein with same and the application thereof. The lead binding protein gene has nucleotide sequences of SEQ ID NO.1 as shown in the specification. The gene is established into recombinant plasmid and transferred into escherichia coli for protein expression, heavy metal enrichment performance of transgenic escherichia coli can be improved, and the gene has great significances for culture of bacteria for specifically and efficiently adsorbing a heavy metal, namely lead.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a lead-binding protein gene, a protein containing it and applications thereof. Background technique [0002] Proteins are the basic components of living organisms, and they are also the executors of various functions of living organisms. The study of protein composition, structure and function will directly clarify the mechanism of changes in living organisms under physiological or pathological conditions. Studies have shown that one-third of the proteins in the human body combine with metal elements to form metalloproteins (including metalloenzymes). In these metalloproteins, metal ions (usually transition metal ions, such as copper, iron, zinc or molybdenum) serve as active centers or cofactors to participate in the completion of protein catalysis, regulation or structural functions. Metal-protein interactions largely determine the function of many proteins ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/24C12N15/31C12N15/70B01J20/22
CPCB01J20/22C07K14/24C12N15/70
Inventor 胡立刚严雪婷陈保卫方临川何滨江桂斌
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI