Culture method of phellinus linteus mycelium and application of phellinus linteus mycelium on health care product
A technique for the fungus and its cultivation method, which is applied in the field of fungal culture, can solve the problems of limiting the utilization and development of the fungus, destroying the growth environment of the fungus, and difficulty in artificial cultivation, etc. Decomposition and utilization of cells, convenient mycelium growth, and the effect of increasing dissolved oxygen
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Embodiment 1
[0017] Take fresh slant strains of Phlomophorus clefthoofus, pick a small amount of strains under aseptic conditions and transfer them to potato dextrose solid medium for activation, and culture them at 28°C for 6 days.
[0018] Take the above-mentioned activated strains, inoculate 13% of the inoculum into the spore-forming medium that has been sterilized under sterile conditions, and cultivate them at 28°C for 8 days. The composition of the sporulation medium is 0.06% of glucose, 5% of corn starch, 5% of bran, 0.65% of potassium dihydrogen phosphate and magnesium sulfate, 0.15% of phenylalanine, 0.09% of cyclic AMP, vitamin B 1 , Vitamin B 12 , Vitamin B 6 The equivalent composition is 0.10%, agar 1.5%, and the balance is water. A large number of conidia were obtained after spore-forming culture. The cultured spore medium was taken to prepare a spore suspension for subsequent operations.
[0019] Take the above-mentioned cultured spore culture medium, inoculate the spore ...
Embodiment 2
[0025] Take fresh slant strains of Phlomophorus clefthoofus, pick a small amount of strains under aseptic conditions and transfer them to potato dextrose solid medium for activation, and culture them at 32°C for 7 days.
[0026] Take the activated strain and inoculate it into the high-temperature sterilized spore-forming medium at an inoculum of 14% under sterile conditions, and cultivate it at 32°C for 9 days. The composition of described sporulation medium is glucose 0.06%, cornstarch 4%, bran 5%, potassium dihydrogen phosphate 0.45% and magnesium sulfate 0.50%, L-glutamine 0.18%, cyclic AMP 0.12%, Vitamin B 1 , Vitamin B 12 , Vitamin B 6 The equivalent composition is 0.09%, agar 1.5%, and the balance is water. The cultured spore medium was taken to prepare a spore suspension for subsequent operations.
[0027]Take the above-mentioned cultured spore culture medium, inoculate the spore suspension into the high-temperature sterilized seed culture medium at an inoculum size...
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