Culture method of phellinus linteus mycelium and application of phellinus linteus mycelium on health care product

A technique for the fungus and its cultivation method, which is applied in the field of fungal culture, can solve the problems of limiting the utilization and development of the fungus, destroying the growth environment of the fungus, and difficulty in artificial cultivation, etc. Decomposition and utilization of cells, convenient mycelium growth, and the effect of increasing dissolved oxygen

Inactive Publication Date: 2018-10-19
广州汉方医学生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The damage to the growth environment of P. clefthoof is increasingly serious, and because of the particularity and complexity of the physiological state of P. clefthoof, the growth cycle is very long, which takes 3 to 4 years, and its growth ha

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take fresh slant strains of Phlomophorus clefthoofus, pick a small amount of strains under aseptic conditions and transfer them to potato dextrose solid medium for activation, and culture them at 28°C for 6 days.

[0018] Take the above-mentioned activated strains, inoculate 13% of the inoculum into the spore-forming medium that has been sterilized under sterile conditions, and cultivate them at 28°C for 8 days. The composition of the sporulation medium is 0.06% of glucose, 5% of corn starch, 5% of bran, 0.65% of potassium dihydrogen phosphate and magnesium sulfate, 0.15% of phenylalanine, 0.09% of cyclic AMP, vitamin B 1 , Vitamin B 12 , Vitamin B 6 The equivalent composition is 0.10%, agar 1.5%, and the balance is water. A large number of conidia were obtained after spore-forming culture. The cultured spore medium was taken to prepare a spore suspension for subsequent operations.

[0019] Take the above-mentioned cultured spore culture medium, inoculate the spore ...

Embodiment 2

[0025] Take fresh slant strains of Phlomophorus clefthoofus, pick a small amount of strains under aseptic conditions and transfer them to potato dextrose solid medium for activation, and culture them at 32°C for 7 days.

[0026] Take the activated strain and inoculate it into the high-temperature sterilized spore-forming medium at an inoculum of 14% under sterile conditions, and cultivate it at 32°C for 9 days. The composition of described sporulation medium is glucose 0.06%, cornstarch 4%, bran 5%, potassium dihydrogen phosphate 0.45% and magnesium sulfate 0.50%, L-glutamine 0.18%, cyclic AMP 0.12%, Vitamin B 1 , Vitamin B 12 , Vitamin B 6 The equivalent composition is 0.09%, agar 1.5%, and the balance is water. The cultured spore medium was taken to prepare a spore suspension for subsequent operations.

[0027]Take the above-mentioned cultured spore culture medium, inoculate the spore suspension into the high-temperature sterilized seed culture medium at an inoculum size...

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PUM

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Abstract

The invention discloses a culture method of phellinus linteus mycelium and a flow of preparing a health care product with the mycelium. The culture method comprises the following steps of activation of a strain of phellinus linteus, spore formation culturing, seed germination and mycelium culturing; in the spore formation culturing stage, amino acid, cyclic adenosine monophosphate and vitamins areserved as growth factors to promote formation of a great amount of spores; and in the mycelium culturing state, mixed powder of plant sawdust crude powder is used as one of components of a culture medium, a wild growth environment of the phellinus linteus is simulated, the plant sawdust crude powder provides a great amount of crude fibers and crude proteins, a solid substrate suitable for growthof the mycelium is provided, and decomposition and utilization of cells are facilitated. Through culturing, the mycelium with good quality and sufficient quantity can be acquired, and good material conditions are created for subsequent preparation of health care nutriments.

Description

technical field [0001] The invention relates to the field of fungal culture, in particular to a method for culturing the mycelium of Phylophorus clefthoofus and a process for preparing health care products with the mycelium. Background technique [0002] The damage to the growth environment of P. clefthoof is increasingly serious, and because of the particularity and complexity of the physiological state of P. clefthoof, the growth cycle is very long, which takes 3 to 4 years, and its growth has certain influence on the external environment. Requirements, so there are few mature fruiting bodies in the wild, but artificial cultivation is very difficult and the conditions are harsh, which largely limits the utilization and development of Ph. [0003] In order to develop a large number of P. clefthoof resources, the scientific community has turned the research direction to P. clefthoof mycelium. Therefore, how to quickly breed P. clefthoof mycelium is an important research topi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L31/00C12R1/645
CPCA23V2002/00A23L31/00C12N1/14A23V2200/328A23V2200/3262
Inventor 涂桂洪王曙聘潘忠林谭志康祝廷霞
Owner 广州汉方医学生物科技有限公司
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