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Human papilloma virus nucleic acid detection kit

A human papillomavirus and detection kit technology, which is applied in the direction of microbial determination/inspection, microorganisms, biochemical equipment and methods, etc., can solve the problems of immaturity of papillomavirus and inability to achieve high-throughput assay.

Inactive Publication Date: 2018-10-19
无锡正则精准医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current fluorescent PCR technology is not mature enough for the detection of human papillomavirus and cannot achieve high-throughput determination.

Method used

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  • Human papilloma virus nucleic acid detection kit
  • Human papilloma virus nucleic acid detection kit
  • Human papilloma virus nucleic acid detection kit

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Embodiment

[0028] A human papillomavirus nucleic acid detection kit, comprising primer AF-F1, primer AF-R1, primer BB-F1, primer BB-R1, probe AF-P1, probe BB-P1, amplification premix, probe Primer premix, and negative and positive quality control, 1), primer sequence design as shown in Table 1:

[0029] Table 1. Primer sequence design

[0030] Primer name

serial number

Sequence 5'-3'

Tm(°C)

reaction tube

Primer AF-F1

SEQ ID NO.1

cag gat tca gag gac gag gaa ga

57℃

B-PPmix

Primer AF-R1

SEQ ID NO.2

tgc agt aca tgt ttg ttt agg tca g

54℃

B-PPmix

Primer BB-F1

SEQ ID NO.3

acg cgc cag acc gtt att g

53℃

B-PPmix

Primer BB-R1

SEQ ID NO.4

ttg gag tca aag tct cca cg

52℃

B-PPmix

Probe AF-P1

SEQ ID NO.5

FAM-tgc cag gaa cag ttg t-MGB

61℃

B-PPmix

Probe BB-P1

SEQ ID NO.6

VIC-tgg gca tat gat aat gat-MGB

56℃

B-PPmix

[0031] 2), sample preparation:...

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Abstract

The invention discloses a human papilloma virus nucleic acid detection kit which is prepared from a primer AF-F1, a primer AF-R1, a primer BB-F1, a primer BB-R1, a probe AF-P1, a probe BB-P1, an amplification premix liquid, a probe primer premix liquid, and negative and position quality control. According to the kit provided by the invention, multiple different fluorescent lights can be adopted for marking the multiple different probes, and an HPV6 type and an HPV11 type can be detected at the same time; the kit can be used for detecting HPV of cervical exfoliated cells; and the detection process after nucleic acid extraction and purification does not exceed 2 hours.

Description

technical field [0001] The invention relates to a human papillomavirus nucleic acid detection kit. Background technique [0002] Human papillomavirus (human papillomavirus, HPV) is an epitheliophilic virus, widely distributed in humans and animals, with a high degree of specificity, and the host of HPV is human. HPV is a general term for a group of viruses, which form a family. Their virus shapes are similar, their DNA restriction enzyme maps are different, and their nucleocapsid proteins have different antigenicities. Spectrum analysis has identified more than 100 types of human papillomavirus so far, and each type is related to specific infection sites and lesions in the body. The genome structure of all HPV viruses is similar, and many HPV types have been discovered so far. With the deepening of research, more new types of HPV will be identified. Human papillomavirus (about 35 types) can infect women's genital tract through various infection routes, thus causing condylo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
CPCC12Q1/6851C12Q1/708C12Q2600/16C12Q2531/113C12Q2545/113C12Q2561/101C12Q2537/143
Inventor 姚鲁帅白静辛君伟王莎
Owner 无锡正则精准医学检验有限公司