Simple device for continuous closed growth of microorganisms and sampling of gas and liquid samples

Abstract

The invention discloses a simple device for continuous closed growth of microorganisms and sampling of gas and liquid samples, belongs to the technical fields of environmental protection and sewage treatment and relates to a reactor device for closed culture of the microorganisms, sewage degradation of the microorganisms and gas-liquid sampling. The device provides a closed environment for continuous culture and sewage degradation of the microorganisms or contaminating materials in a culture medium, and provides possibility for determining gas components produced or consumed by the microorganisms; meanwhile, the concentration of the contaminating materials volatizing from sewage can be determined, and the problem that the measuring result is inaccurate due to concentration reduction in a water body caused by volatilization of the contaminating materials; besides, the device disclosed by the invention can conveniently sample the gas-liquid samples and facilitates operation and determination; the device has the advantages of simple structure, low cost and convenience in observation.

Description

technical field [0001] The invention belongs to the technical field of microbial culture and sewage treatment in environmental protection, and discloses a simple microbial reaction device, in particular to a reactor device used for microbial airtight culture or microbial degradation of sewage and gas-liquid sampling. Background technique [0002] With the rapid growth of China's economy, the industrial process continues to move forward. At the same time, the generated industrial wastewater is also increasing year by year. If it is not treated, it will seriously pollute the environment, destroy the ecological balance, and affect the health and safety of human beings. Survive. Moreover, the characteristics of industrial wastewater gradually include: high COD value, coexistence of various pollutants, and volatile or semi-volatile organic pollutants such as polycyclic aromatic hydrocarbons and organic pesticides are increasing year by year. Microorganisms such as bacteria and m...

AI Technical Summary

Problems solved by technology

The common experiments for continuous culture of microorganisms or microbial degradation in the laboratory are to inoculate the microorganisms in conical flasks and place the conical flasks in a constant temperature shaking incubator for cultivation. Under this condition, the culture solution or degradation solution is directly Connected with the air, volatile or semi-volatile pollutants are easily volatilized into the air, resulting in inaccurate measurement results, or when measuring the gas produced or consumed by the cultured microorganisms, such as CO 2 or O 2 When the culture device communicated with the air is difficult to meet the experimental measurement requirements, so a simple and airtight reaction device is extremely important

However, it is not enough to meet the above conditions. In many experiments, it is necessary to take samples from the reaction device regularly to measure the growth of microorganisms in the solution or the content of pollutants to be degraded in the aqueous solution, and to measure the gas content in the device. The device does not consider this point, such as patent No. CN2683656Y, which is named as a continuous culture experimental device for microorganisms. Compared with traditional reactors, it has the advantages of airtight conditions, simple structure, and easy observation, but it is still very difficult to achieve gas Liquid sample sampling operation

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