A kind of production technology of Cordyceps sinensis mycelium and fruiting body

A production process and technology of Cordyceps sinensis, which is applied in the field of production process of cordyceps sinensis mycelium and fruiting body, can solve the problems of high risk, easy infection of miscellaneous bacteria, difficult symbiosis temperature, etc., and achieve the effect of no toxic and side effects

Active Publication Date: 2020-05-19
SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the symbiotic inoculation of the two liquid species on the same medium has an insurmountable defect: since Cephalosporium is a low-temperature bacterium and I. cephalosporin is a normal-temperature bacterium, it is difficult to effectivel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The separate gathering of embodiment 1 mycelia

[0029]In this embodiment, the harvesting steps of the mycelia include: 1) stirring and mixing 360g oats, 200g corn dregs, 200g silkworm chrysalis powder, and 200g whole eggs at room temperature to obtain a solid medium; 2) mixing 90g White sugar, 0.045g potassium dihydrogen phosphate, 0.0225g magnesium sulfate, 0.009gV B1 After mixing, add water to 900ml and stir and mix evenly at room temperature to obtain a nutrient solution; 3) mix the solid medium with the nutrient solution so that the nutrient solution is completely absorbed by the solid medium, and then divide into 30g of dry material per bottle Put it into 30 canned bottles, then sterilize it at 120°C and 0.15MPa for 1 hour, when the sterilized main material is cooled to 20°C, insert 1.5g / bottle into the bottle Solid species; 4) Cultivate the inoculated canned bottle at 0°C-16°C in the dark for 35 days. When the surface of the medium is covered with hyphae and ext...

Embodiment 2

[0030] Embodiment 2 gathers mixed product (fruiting body and mycelium do not separate)

[0031] In this example, the initial steps of harvesting the mixture are the same as step 1) to step 3) in Example 1, the difference is that when the inoculated cans are incubated at 0°C-16°C in the dark After 35 days, when the surface of the medium is covered with hyphae and extends downward, add 3ml of liquid medium to each bottle, insert 0.045g of Paecilomyces liquid species, and culture at a symbiotic temperature of 15°C for 35 days until fruiting bodies When it grows to about 1cm and the bottle is covered with mycelium and all the mycelium turns taupe, it can be harvested, dried, crushed and packaged to prevent moisture and pollution. In this embodiment, the total amount of the harvested product is 450 g, and the biotransformation rate is 50%. It is detected that the content of adenosine in the harvested product is 0.11%, and the content of cordycepin is 0.1%.

Embodiment 3

[0032] The independent gathering of embodiment 3 fruiting bodies

[0033] In this embodiment, the initial steps of harvesting the fruiting body are the same as in Example 2, the difference being that when the culture in Example 2 grows fruiting body, the culture is placed in the culture room, and the % humidity, daytime temperature of 21°C and nighttime temperature of 10°C. During the cultivation process, the astigmatism with an illumination of 200LX-1000LX is alternately illuminated for each light segment for 4 hours, and ventilated once a day in the morning and evening. Second half an hour, until the white hyphae all turn taupe, the fruiting body grows to 3cm-4cm high and is about to emit spores and gather when actually not emitting, dry and pack. In this embodiment, the content of adenosine and cordycepin in the harvested dried fruit body is 0.056%.

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PUM

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Abstract

The invention discloses a production process of ophiocordyceps sinensis mycelia and sporocarps. The production process includes the steps of 1, culturing hirsutella rhossiliensis mycelia; 2, conducting symbiotic sprouting; 3, culturing the sporocarps. During culturing of the hirsutella rhossiliensis mycelia, solid culture media and a nutrient solution are prepared; the solid culture media are uniformly mixed with the nutrient solution and then placed in culture vessels respectively, hirsutella rhossiliensis solid seeds are put into the culture vessels after sterilization treatment and cultureduntil the surfaces of the culture media are covered with hyphae. During symbiotic sprouting, liquid culture media are prepared and added into the culture vessels in step 1 under an aseptic condition;paecilomyces varioti liquid seeds are put into the culture vessels and cultured until the culture media are covered with two kinds of hyphae and buds of the sporocarps grow out. During culturing of the sporocarps, cultured objects obtained in step 2 are placed in a culture room for culturing until the hyphae turn grey brown, and harvesting is carried out when the sporocarps are about to release spores.

Description

technical field [0001] The invention relates to a production process of Cordyceps sinensis mycelium and fruiting body, in particular to a production process of using Paecilomyces as an associated bacterium and accompanying Mortierella to produce the Cordyceps sinensis mycelium and fruiting body, belonging to the technical field of Cordyceps sinensis production. Background technique [0002] Since the 1980s, scientific and technical personnel have isolated more than 20 kinds of fungi from natural Cordyceps sinensis, except for Trichosporum, which is a fungus of Cordyceps sinensis, the rest are all associated fungi. Under different external conditions, these associated bacteria enter the host with their respective identities first or later, coexist with the hairy sporophyte in the host and compete for growth. During the coexistence and competition growth process, two or more fungi form a composite Forms, dynamic coexistence and the reproduction of sexual generations of Cordyce...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/00A01G18/20
CPCA01G18/00A01G18/20A01G18/40
Inventor 杨毅周翔胡素花刘巧云周文贵刘超英
Owner SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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