Method for dissolving low-grade phosphate rock powder by utilizing endophytic bacteria of myriophyllum spicatum
A technology of foxtail algae and endophyte, which is applied in the directions of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of single, low-grade phosphate rock, etc., and achieves a simple process, is conducive to reproduction, and improves dissolution efficiency. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0032] A method for dissolving low-grade phosphate rock powder by endophytic bacteria of P. spicaculata, comprising the steps of:
[0033] (1) Isolation and screening of endophytes:
[0034] a. Isolation of endophytic bacteria from P. spicaculata:
[0035] In the field, collect the intact and vigorously grown Trichosanthes spicaris, rinse it with tap water, and then rinse it with sterile water 10 times, blot it dry with sterile filter paper, soak it in 70% ethanol solution for 5 minutes, and then soak it in 1.2% ethanol solution for 5 minutes. Soak in sodium hypochlorite solution for 3 minutes, soak in 0.1% mercuric chloride solution for 2 minutes, and finally rinse with sterile water for 10 times and blot dry. Cut the plant into 1.5cm sections, cut them longitudinally, and stick the cut surface on the beef Paste the surface of the peptone solid medium, and place the prepared plate in a constant temperature incubator at 25°C for 2 weeks to obtain bacterial cells;
[0036] b....
Embodiment 2
[0046] A method for dissolving low-grade phosphate rock powder by endophytic bacteria of P. spicaculata, comprising the steps of:
[0047] (1) Isolation and screening of endophytes:
[0048] a. Isolation of endophytic bacteria from P. spicaculata:
[0049] In the wild, collect the complete and vigorously grown Trichosanthes spicaris, rinse it with tap water, and then rinse it with sterile water 8 times, blot it dry with sterile filter paper, soak it in 70% ethanol solution for 3 minutes, and then soak it in 1% ethanol solution for 3 minutes. Soak in sodium hypochlorite solution for 2 minutes, soak in 0.1% mercuric chloride solution for 1 minute, and finally rinse with sterile water for 8 times and blot dry. Cut the plant into 1 cm sections, cut them longitudinally, and stick the cut surface on the beef paste On the surface of peptone solid medium, place the prepared plate in a constant temperature incubator at 25°C for 2 weeks to obtain bacteria;
[0050] b. Screening of endop...
Embodiment 3
[0060] A method for dissolving low-grade phosphate rock powder by endophytic bacteria of P. spicaculata, comprising the steps of:
[0061] (1) Isolation and screening of endophytes:
[0062] a. Isolation of endophytic bacteria from P. spicaculata:
[0063] In the wild, collect the complete and vigorously grown Trichosanthes spicaris, rinse it with tap water, and then rinse it with sterile water 9 times, blot it dry with sterile filter paper, soak it in 70% ethanol solution for 3 minutes, and then soak it in 0.8% ethanol solution for 3 minutes. Soak in sodium hypochlorite solution for 2 minutes, soak in 0.1% mercuric chloride solution for 1 minute, and finally rinse with sterile water for 9 times and blot dry. Cut the plant into 0.8 cm sections, cut them longitudinally, and stick the cut surface on the beef On the surface of the peptone solid medium, place the prepared plate in a constant temperature incubator at 25°C for 13 days to obtain the bacteria;
[0064] b. Screening ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com