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A strain of Rhizopus oryzae with salt tolerance and its application

A technology of Rhizopus oryzae and saline-alkali land, applied in the field of agricultural microorganisms and ecological restoration, can solve the problems of reducing the ecological restoration effect of microorganisms on saline-alkali land, difficult realization of target strains, and unfavorable survival of microorganisms, so as to achieve good salt tolerance and improve tolerance Effect of strong saltiness and survivability

Active Publication Date: 2021-05-04
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of microorganisms is an indicator of soil fertility or saline-alkali land, but the number of microorganisms in saline-alkali land is extremely small. The main reason is that the saline-alkali environment is not conducive to the survival of microorganisms. High-concentration salt ions cause dehydration of microbial cells and inhibit metabolism, which not only inhibits microbial growth. growth, and reduce the ecological restoration effect of microorganisms on saline-alkali land
Bacterial strains are the basis for the biological improvement of saline-alkali land with microorganisms. At present, the bottleneck restricting the development of saline-alkali land biological improvement is mainly the problem of breeding high-efficiency strains. Since the number of microbial flora in saline-alkali soil is relatively small, it is necessary to isolate the target bacteria This is extremely difficult to achieve, especially isolating strains that can tolerate high salt
However, there is no report on the salt tolerance of Rhizopus oryzae

Method used

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  • A strain of Rhizopus oryzae with salt tolerance and its application
  • A strain of Rhizopus oryzae with salt tolerance and its application
  • A strain of Rhizopus oryzae with salt tolerance and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and identification of bacterial strains

[0033] 1. Isolation of strains:

[0034] (1) Primary screening:

[0035] Weigh 1g soil sample (collected from alfalfa rhizosphere soil in Hekou District, Dongying City, Shandong Province) in 100ml sterile water, place in a shaker at 30°C at 150rpm for 10min, and take 100μl 10 -2 The diluted solution was spread on a PDA medium plate with a salt concentration of 2%, and cultured at 30°C until colonies grew out.

[0036] (2) Re-screening:

[0037] The colonies obtained by the primary screening were separated and inoculated into medium with higher salt concentration by streaking on the plate, and the concentration increased according to the gradient. The strain that grew vigorously at the highest concentration was isolated and purified by streaking.

[0038] Wherein, the formula of PDA medium: peeled potato 200g, glucose 20g, agar 14g, distilled water 1000ml.

[0039] After screening, a salt-tolerant strain...

Embodiment 2

[0055] Embodiment 2: pot experiment

[0056] 1. Preparation of bacterial agent:

[0057] Inoculate the Rhizopus oryzae with the preservation number CGMCC NO.15267 on the PDA medium, and after 30°C, 150rpm shake flask culture for 48h, when the thalline grows to the logarithmic growth phase, the thalline is collected by centrifugation at 8000rpm and washed with sterile water. After washing the bacterium twice, the OD value of the bacterium is adjusted to 0.5-0.6 with sterile water, and the bacterial agent is prepared.

[0058] 2. Pot experiment:

[0059] Mix nutrient soil and vermiculite at a volume ratio of 4:1 to obtain a potting substrate, then sterilize at 121°C for 1 hour, and pack it into potted flowerpots (9cm long x 9cm wide x 9cm high) after cooling, about 200g per pot .

[0060] Select alfalfa seeds with full particles and the same size, put them into sterilized soil for seedling cultivation after surface disinfection, and select seedlings with consistent growth aft...

Embodiment 3

[0064] Embodiment 3: the improvement effect of rhizopus oryzae (Rhizopus oryzae) to saline-alkali soil

[0065] 1. Test location: The test was carried out in the saline-alkali land of the Yellow River Delta from June 2017 to November 2017.

[0066] 2. Test materials: the repair matrix and alfalfa seeds formed by inoculating Rhizopus oryzae screened and isolated in Example 1 into sawdust and cultured for 14 days.

[0067] 3. Experimental design: The area of ​​the experimental field is one mu, and two treatments are set up, namely:

[0068] Treatment 1: sawdust applied only;

[0069] Treatment 2: Restoration matrix of salt-tolerant bacteria.

[0070] 4. Test method: Divide one mu of land into two plots on average, and apply improved substrate to each plot according to the experimental design, 60 kg per mu.

[0071] The pH value of the soil was measured before and after the test, as well as the composition of soil aggregates after the test, and the activities of urease, catala...

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Abstract

The invention discloses a strain of Rhizopus oryzae, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 22, 2018, and its biological preservation number is: CGMCC NO.15267. The rhizopus oryzae (Rhizopus oryzae) obtained by screening and separating in the present invention has good salt tolerance, strong survivability under harsh conditions, especially high-salt concentration environment, fast growth speed, and rapid mass colonization in plant rhizosphere soil. Soil improvement in saline-alkali land. It can significantly slow down the symptoms of salt damage under salt stress, increase the biomass of plants to promote their growth, and help to improve the salt tolerance of plants.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms and ecological restoration, in particular to a rhizopus oryzae with salt tolerance and application thereof. Background technique [0002] Soil salinization is one of the main abiotic stress factors that threaten global agricultural production and crop yields. At present, nearly 50% of the irrigated land in the world is threatened by different degrees of salt stress. In my country, there are about 9.9×10 7 hm 2 There is a problem of salinization hazards in the land, and the threatened area is increasing year by year, leading to regional ecological deterioration. Under the premise of promoting ecological benefits, biological improvement measures have become a research hotspot. [0003] At present, the main biological improvement measures include cultivating and planting salt-tolerant crops, and research on salt-tolerant transgenic plants, but such methods often take a long time...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10C05F11/08C12R1/845
CPCB09C1/105C05F11/08C12N1/14C12N1/145C12R2001/845
Inventor 娄燕宏诸葛玉平宋付朋王会崔秀敏孙鑫
Owner SHANDONG AGRICULTURAL UNIVERSITY
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