Platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-mode imaging function as well as preparation method and application of platinum sulfide protein nanoparticle

A multi-modal imaging, near-infrared light technology, applied in medical preparations containing active ingredients, photodynamic therapy, echo/ultrasonic imaging agents, etc., to achieve the effects of convenient preparation, flexible use, and strong X-ray attenuation ability

Pending Publication Date: 2018-11-09
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no report of platinum sulfide used in photothermal therapy, and there is no nano-reagent loaded with a platinum compound in the prior art that can simultaneously play photothermal effect and modern imaging diagnosis of four modalities. Effect

Method used

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  • Platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-mode imaging function as well as preparation method and application of platinum sulfide protein nanoparticle
  • Platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-mode imaging function as well as preparation method and application of platinum sulfide protein nanoparticle
  • Platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-mode imaging function as well as preparation method and application of platinum sulfide protein nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation and Application of Nanoparticles

[0052] 1. Preparation of nanoparticles: Weigh 20.0 mg of human serum albumin (HAS, molecular weight 66KD) and dissolve it in 10.0 mL of deionized water, weigh 2.7 mg of platinum dichloride (PtCl 2 , molecular weight 265.99) dissolved in 2 mL of deionized water to the solution, weighed 9.6 mg of sodium sulfide (Na 2 S·9H 2 O, molecular weight 240.18) was dissolved in 0.5 mL deionized water. Slowly add platinum dichloride aqueous solution to the protein solution and vigorously stir to mix the two thoroughly, then add sodium sulfide aqueous solution to the solution. The molar ratio of Pt:S in the solution is 1:4, and the volume ratio of protein solution, platinum dichloride solution and sodium sulfide solution is 1:0.2:0.05. Place the solution in a 55°C water bath and stir vigorously for 4 hours. After the reaction is complete, place the reaction product in a dialysis bag (molecular weight cut-off 3500), dialyze w...

Embodiment 2

[0091] When preparing platinum sulfide protein nanoparticles in Example 1, the concentration of human serum albumin was adjusted to 4 and 8 mg / mL respectively (in Example 1, the protein concentration was 2 mg / mL), and other steps were the same as in Example 1 , two kinds of nanoparticles with sizes of 3.2 ± 0.2 nm and 4.5 ± 0.4 nm can be prepared respectively, and PtS-NDs with a concentration of 1.0 mM can be obtained at (785 nm, 1.5 W cm -2 ) irradiation for 5 min, the temperature of the solution can be increased by 16°C and 18.5°C, respectively, and the photothermal conversion efficiency is 28.7% and 31.2%, respectively.

Embodiment 3

[0093] The reaction time in the preparation process of platinum sulfide protein nanoparticles in Example 1 was adjusted to 1 h (in Example 1, the reaction time was 4 h), the absorption in the near infrared region reached the maximum and remained stable, and the size of about 4.5 nm can be prepared. , PtS-NDs at a concentration of 1.0 mM at (785 nm, 1.5 W cm -2 ) within 5 minutes of irradiation can increase the solution temperature by 19.5°C, and the photothermal conversion efficiency is 31.8%.

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Abstract

The invention discloses a platinum sulfide protein nanoparticle with a near-infrared photothermal effect and a multi-mode imaging function as well as a preparation method and application of the platinum sulfide protein nanoparticle. By carrying out formulation screening and process limitation, the platinum sulfide protein nanoparticle with the near-infrared photothermal effect and the multi-mode imaging function is prepared in a water phase, has an ultra-small particle size and good stability, tumor targeting property and photothermal effect and is integrated with near-infrared imaging, CT imaging, photoacoustic imaging and thermal imaging functions, the high-sensitivity and high-resolution precise localization of tumors is realized, an efficient photothermal effect is generated under thestimulation of near-infrared light, tumor cells are killed through thermal ablation, and the efficient, safe, visual and accurate treatment of the tumors is realized; and the platinum sulfide proteinnanoparticle has potential to be further applied to the clinic.

Description

technical field [0001] The invention discloses an ultra-small platinum sulfide protein nanoparticle with near-infrared photothermal effect and multimodal imaging function and high drug loading capacity, as well as a preparation method and application thereof. Background technique [0002] Malignant tumors are one of the major malignant diseases that seriously threaten human health, and their morbidity and mortality are on the rise both at home and abroad. How to achieve accurate diagnosis and efficient treatment of tumors is the focus and difficulty of current research. Since temperature is an important parameter that affects the activity of enzymes and the rate of various biochemical reactions in the body, an elevated body temperature usually indicates infection or other diseases. Through external near-infrared light irradiation, nanomaterials loaded with photothermal reagents entering tumor cells absorb light and convert it into heat, which can lead to cell apoptosis or e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61K49/22A61K49/04A61P35/00
CPCA61K41/0052A61K41/0057A61K49/00A61K49/0045A61K49/0093A61K49/04A61K49/0485A61K49/221A61K49/225A61P35/00
Inventor 杨红陈华兵王雪柯亨特李明徐涛张米娅
Owner SUZHOU UNIV
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