Acinetobacter CL04 and an application thereof in village sewage phosphorus-removing processing

A phosphorus treatment, CCTCCM2017541 technology, applied in Acinetobacter CL04 and its application in the phosphorus removal treatment of village sewage, can solve the problems of low content of phosphorus accumulating bacteria, inability to effectively reproduce and efficiently absorb phosphorus, and effluent phosphorus exceeding the standard, etc. The effect of high-efficiency phosphorus removal effect

Active Publication Date: 2018-11-09
ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the content of phosphorus-accumulating bacteria in ordinary sewage treatment equipment is too low and the effect of phosphorus-accumulating bacteria is very poor. They cannot effectively reproduce into dominant bacteria and efficiently absorb phosphorus in the sewage solution, and the phosphorus in the effluent seriously exceeds the standard.

Method used

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  • Acinetobacter CL04 and an application thereof in village sewage phosphorus-removing processing
  • Acinetobacter CL04 and an application thereof in village sewage phosphorus-removing processing
  • Acinetobacter CL04 and an application thereof in village sewage phosphorus-removing processing

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Experimental program
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Effect test

Embodiment 1

[0025] 1. Screening of phosphorus accumulating bacteria

[0026] 1.1 Sampling: Take 5g of river silt from Donghu Campus of Zhejiang A&F University.

[0027] 1.2 Enrichment culture: add the sample to 100ml LB liquid medium, shake at 37°C and 200rpm for 60min. Take 10ml of the shaken sample and add 100ml of enrichment medium, first insert the enrichment medium with the lowest concentration of potassium dihydrogen phosphate, culture on a shaker at 37°C and 200rpm for 3 days, and then transfer to the concentration of potassium dihydrogen phosphate The higher enrichment medium was continued to be cultivated according to the above conditions, and the enrichment was carried out in turn until the enrichment medium of 4 concentrations were all cultured.

[0028] 1.3 Purification: After the enrichment is completed, the enriched bacterial liquid is gradiently diluted to an appropriate concentration and spread on an LB plate, and cultured at 37°C for 12-24 hours.

[0029] 1.4 Coarse sie...

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Abstract

The invention provides a strain of Acinetobacter CL04 and an application thereof. A bacterial strain has the advantages of effective phosphorus-accumulating and becomes a dominant bacteria in sewage processing devices, phosphorus in the sewage can be absorbed in a large number, polyphosphate is formed in vivo, and the purpose of removing the phosphorus is achieved through exhausting bacterial sludge deposition. The bacterial strain can be well integrated into the current common biological method sewage processing technology, is suitable for village, city life sewage, is also suitable for sewage and waste water of aspects like river, business and industry, change is not required or improvement cost is lower, and phosphate removing has wide application value in the sewage processing devices(such as village purifying tanks, city sewage treatment plants, and the like.

Description

technical field [0001] The invention relates to a new strain of bacteria—acinetobacter CL04Acinetobacter sp.CL04, and the application of the strain to remove phosphorus in sewage. Background technique [0002] The intensification of human activity has brought enormous pressure to the ecological environment, especially a large amount of industrial, agricultural and domestic sewage not only brought great pressure to sewage treatment, but also caused phosphorus to flow into various water bodies, resulting in eutrophication of water bodies. At present, the treatment of phosphorus in sewage treatment mainly includes chemical flocculation and sedimentation method and microbial phosphorus removal. Microbial phosphorus removal is safe, non-toxic, low-cost, economical and practical. The chemical method is mainly to add aluminum salts, iron salts and phosphorus to produce insoluble precipitates, while the microbial method is mainly to facilitate the absorption of phosphorus in sewage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
CPCC02F3/34C02F2101/105C12N1/20C12N1/205C12R2001/01
Inventor 郑展望潘碧文崔志文谢柳金鹏
Owner ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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