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Application of substance for detection of KIAA0125 gene expression level in assistant identification of ALL (acute lymphocytic leukemia)
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A gene expression level, acute lymphocyte technology, applied in the application field of auxiliary identification of acute lymphoblastic leukemia, can solve the problem of malignant tumors that have not yet been seen
Active Publication Date: 2018-11-16
PEOPLES HOSPITAL PEKING UNIV
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At present, there are no related reports on KIAA0125 gene and hematological malignancies
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Embodiment 1
[0046] Embodiment 1, detect the complete set of reagents and sensitivity detection of KIAA0125 gene expression level
[0047] 1. Assembling the set of reagents
[0048] This example provides a kit of reagents for the KIAA0125 gene (NCBI gene bank sequence number: NR_026800.2, sequence 7 in the sequence listing), which can be used to detect the expression level of the KIAA0125 gene. Composed of upstream primer KIAA0125-FP and downstream primer KIAA0125-RP, the amplification product is 119bp) and probe A (probe KIAA0125-probe):
[0049] Upstream primer KIAA0125-FP: 5'-GGGAGGTCAAGAGTTGGAGCA-3' (sequence 1);
[0050] Downstream primer KIAA0125-RP: 5'-CATCCCTTCCCAATGTCCGT-3' (sequence 2);
[0051] Probe A KIAA0125-probe: 5'-FAM-TCAGTTCTGACAGGGCCTCAGACCTAAGG-BHQ1-3' (SEQ ID NO: 3).
[0052] In the complete set of reagents in this example, the upstream primer KIAA0125-FP, the downstream primer KIAA0125-RP and the probe A are packaged independently, and the molar ratio between the ...
Embodiment 2
[0062] Embodiment 2, detect leukemia patient and cell line
[0064] The kit consists of the following components: the complete set of reagents in Example 1 and the primer pair B and probe B for the ABL1 gene (NCBI gene bank sequence number: NM_005157.5, sequence 8 in the sequence table) as an internal reference gene, and the primer pair B Consists of upstream primer ABL1-F and downstream primer ABL1-R:
[0065] Upstream primer ABL1-F (sequence 4 of the sequence listing): 5'-TGGAGATAACACTCTAAAGCATAACTAAAGGT-3';
[0066] Downstream primer ABL1-R (sequence 5 of the sequence listing): 5'-GATGTAGTTGCTTGGGACCCA-3';
[0067] Probe B ABL1-T-probe (5'→3'):
[0068] FAM-CCATTTTTGGTTTGGGCTTCACACCATT-TAMRA (the nucleotide sequence is SEQ ID NO: 6 in the Sequence Listing).
[0069] 2. Use the kit in step 1 to detect cell lines
[0070] Samples to be tested: mononuclear cells (mononuclear cells 1 and 2) from two healthy volunteers, BV-173, BALL-1 from Guangzhou...
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Abstract
The invention discloses an application of a substance for detection of KIAA0125 gene expression level in assistant identification of ALL (acute lymphocytic leukemia). It is found that the KIAA0125 gene expression level is related to ALL, and the KIAA0125 gene expression level in patients with ALL is significantly lower than that of healthy people. The KIAA0125 gene expression level is also associated with the therapeutic effect of patients with ALL. The KIAA0125 gene expression level in healthy people is significantly higher than that in first-visit patients with ALL and remission patients with ALL, and the IAA0125 gene expression level in the remission patients with ALL is significantly higher than that in the first-visit patients with ALL. The IAA0125 gene expression level can be used for predicting attack and therapeutic effect of the patients with ALL.
Description
technical field [0001] The invention relates to the application of a substance for detecting the expression level of KIAA0125 gene in assisting identification of acute lymphoblastic leukemia in the field of biotechnology. Background technique [0002] Acute lymphocytic leukemia (ALL) is a malignant blood tumor originating from the lymphatic system. The main clinical manifestations are increased lymphocyte count, anemia, skin and gum bleeding, hepatosplenomegaly, and central nervous system infiltration. At present, although the prognosis has been significantly improved by new drug therapy and hematopoietic stem celltransplantation, there are still some children and adults with ALL, and the outcome of recurrence and death is inevitable. The clinical heterogeneity of ALL is extremely high, and abnormal gene markers are the basis for the classification of different disease subtypes. However, about 10-30% of childhood ALL and 30-50% of adult ALL lack molecular markers related ...
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