Efficient hydrolyzed composite bacterium group of Baijiu waste spent grains

A technology of compound bacteria and hydrolysis bacteria, which is applied in the field of screening and research of complex bacteria groups, can solve the problems of low usable value, polluted environment, low nutrition, etc., and achieve the effect of promoting development and utilization

Inactive Publication Date: 2018-11-20
GUIZHOU MEDICAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention: In view of the situation of high acidity, low nutrition, low usable value, and environmental pollution of discarded grains, provide a kind of high-efficiency microbial group to improve the hydrolysis rate of discarded grains, reduce the storage space of waste residues, reduce the The technical solution for waste residue treatment costs promotes the maintenance of a good ecological environment, achieves sustainable recycling of liquor waste waste, and lays the foundation for the government's goal of zero emissions and pollution-free advocacy of enterprises

Method used

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  • Efficient hydrolyzed composite bacterium group of Baijiu waste spent grains
  • Efficient hydrolyzed composite bacterium group of Baijiu waste spent grains
  • Efficient hydrolyzed composite bacterium group of Baijiu waste spent grains

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Embodiment Construction

[0031] First, the Westerdijk Research Center purchases the fungi that hydrolyze the distilled grains of liquor, and isolates the fungal strains from the acidic environment of the distilled grains;

[0032] 1. Sampling in the acidic environment of the liquor enterprise (collecting liquor dregs and fresh liquor dregs in the winery, distiller dregs are used to isolate fungi that may have hydrolyzed liquor dregs, fresh liquor dregs are used to add into the culture medium), weigh 10 g of compost samples into a 250 mL Erlenmeyer flask, add 100 mL of sterile water, and shake in a 30°C constant temperature shaking box for 3 to 4 hours.

[0033] 1.1 Isolation of strains: Let the uniformly shaken bacterial solution stand for 30 minutes, draw an appropriate amount of supernatant for 10-fold gradient dilution, and draw 10 -1 ~10 -5 Inoculate 0.5 mL of each gradient bacterial solution on YPD medium (yeast extract peptone glucose agar medium, containing chloramphenicol), and incubate at 28...

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Abstract

The invention discloses an efficient hydrolyzed composite bacterium group of Baijiu waste spent grains. The efficient hydrolyzed composite bacterium group of the white spirit waste spent grains is characterized in that an efficient hydrolyzed bacterial strain is one or the composition of more than one of CBS 113.65 Oidiodendron echinulatum, CBS 246.84 Phanerochaete chrysosporium, CBS 383.78 Trichoderma reesei, CBS 554.65 Aspergillus niger, CBS 110.42 Aspergillus niger and GYM-37 Bjerkandera adusta.

Description

technical field [0001] The present invention relates to the optimization research of the scheme of hydrolyzing liquor and discarding grains, in particular to the screening research of two groups of new high-efficiency hydrolyzing liquor and discarding grains compound bacterial groups. technical background [0002] Liquor distilled grains are the husks and residues of grains that are poor in nutrition after repeated fermentation and distillation to extract alcohol. That is, the content of distiller's grains is high in moisture and lignocellulose (cellulose, hemicellulose, lignin), and the content of crude protein and crude fat is low. Because the lost grains have less nutrients and lignocellulose is difficult to hydrolyze, it cannot be as effective as ordinary distiller's grains Therefore, the transformation of waste waste is greatly restricted. Moreover, the waste waste has high acidity and high moisture content, which is very easy to spoil and deteriorate, causing pollutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/66C12R1/885C12R1/645
CPCC12N1/14
Inventor 康颖倩罗鹏
Owner GUIZHOU MEDICAL UNIV
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