Protein preservation liquid, preparation method thereof, and method used for preservation of proteins using protein preservation liquid
A technology of preservation solution and protein, which is applied in the field of protein preservation to save the time required for thawing, facilitate detection or test operation, and reduce costs
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Embodiment 1
[0058] Example 1: Protein preservation solution, based on the preparation of 100mL preservation solution, the components included and their corresponding dosages are shown in Table 1, and were prepared through the following steps:
[0059] Step 1, prepare Tris-HCl buffer solution:
[0060] At 18-28°C, add 42mL of Tris into 30mL of pure water, mix thoroughly, adjust the pH value to 7.4 with HCl with a concentration of 2mol / L, add pure water to 100mL, and obtain Tris-HCl buffer solution;
[0061] Step 2, prepare casein solution:
[0062] Step A, at 18-28°C, according to parts by weight, weigh 6 parts of casein, add 45 parts of water, and mix thoroughly to form the first mixture;
[0063] Step B, adding 20 parts of NaOH with a concentration of 2mol / L to the first mixture obtained in step A, and fully mixing to form a second mixture;
[0064] Step C, adding 10 parts of Tris-HCl buffer solution with a concentration of 2mol / L to the second mixture obtained in step B, and fully mixin...
Embodiment 2-5
[0070] Example 2-5: protein preservation solution, the difference from Example 1 is that, based on the preparation of 100mL preservation solution, the components included and their corresponding dosages are shown in Table 1.
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