Protein preservation liquid, preparation method thereof, and method used for preservation of proteins using protein preservation liquid

A technology of preservation solution and protein, which is applied in the field of protein preservation to save the time required for thawing, facilitate detection or test operation, and reduce costs

Inactive Publication Date: 2018-11-30
杭州百殷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the prior art, the first purpose of the present invention is to provide a protein preservation solution, which solves the disadvantages of traditional preservation solutions that need to be stored at extremely low temperatures, and has the ability to stably preserve proteins and make protein detection results more accurate. Effect

Method used

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  • Protein preservation liquid, preparation method thereof, and method used for preservation of proteins using protein preservation liquid
  • Protein preservation liquid, preparation method thereof, and method used for preservation of proteins using protein preservation liquid
  • Protein preservation liquid, preparation method thereof, and method used for preservation of proteins using protein preservation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Protein preservation solution, based on the preparation of 100mL preservation solution, the components included and their corresponding dosages are shown in Table 1, and were prepared through the following steps:

[0059] Step 1, prepare Tris-HCl buffer solution:

[0060] At 18-28°C, add 42mL of Tris into 30mL of pure water, mix thoroughly, adjust the pH value to 7.4 with HCl with a concentration of 2mol / L, add pure water to 100mL, and obtain Tris-HCl buffer solution;

[0061] Step 2, prepare casein solution:

[0062] Step A, at 18-28°C, according to parts by weight, weigh 6 parts of casein, add 45 parts of water, and mix thoroughly to form the first mixture;

[0063] Step B, adding 20 parts of NaOH with a concentration of 2mol / L to the first mixture obtained in step A, and fully mixing to form a second mixture;

[0064] Step C, adding 10 parts of Tris-HCl buffer solution with a concentration of 2mol / L to the second mixture obtained in step B, and fully mixin...

Embodiment 2-5

[0070] Example 2-5: protein preservation solution, the difference from Example 1 is that, based on the preparation of 100mL preservation solution, the components included and their corresponding dosages are shown in Table 1.

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Abstract

The invention discloses a protein preservation liquid, a preparation method thereof, and a method used for preservation of proteins using the protein preservation liquid, and belongs to the technicalfield of protein preservation. 100mL of the protein preservation liquid comprises 2.535 to 3.501g of sodium chloride, 6 to 7mL of Tris-HCl buffer, 20 to 28mL of casein solution, 28 to 31<mu>L of an antiseptic, and the balance pure water. The pH value of the Tris-HCl buffer is 7.4 to 7.5. The protein preservation liquid is capable of avoiding a defect of convention preservation liquid that an extremely low temperature is needed for preservation, can be used for stable protein preservation, and is capable of obtaining relatively accurate protein preservation results.

Description

technical field [0001] The invention relates to the technical field of protein preservation, more specifically, it relates to a protein preservation solution and a preparation method thereof, and a method for preserving protein by using the preservation solution. Background technique [0002] There are many kinds of proteins, and they are easily denatured or degraded. Therefore, it is difficult to preserve proteins. [0003] Considering that the protein is easily denatured or degraded in the preservation solution, resulting in the destruction of the three-dimensional structure of the protein, the traditional method of protein preservation is usually to preserve the protein in the preservation solution, and place the preservation solution with the protein at an ultra-low temperature. Store frozen. [0004] However, when detecting the preserved protein, it is necessary to melt the existing preservation solution, and then take out part of the preserved protein for detection. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/00
CPCC07K1/00
Inventor 许安庆刘恒生汪鹏
Owner 杭州百殷生物科技有限公司
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