Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of anti-duck reovirus yolk antibody and its preparation method and application

A technology of reovirus and yolk antibody, applied in the field of yolk antibody against duck reovirus and its preparation, can solve the economic loss of meat duck breeding duck breeding industry, restrict the development and application of yolk antibody, and breed duck egg production and meat duck weight loss and other problems, to achieve the effect of preventing and treating infection, good commercial development prospects, and reducing the risk of adverse reactions

Active Publication Date: 2020-10-23
SHANDONG AGRICULTURAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since 2017, a large-scale outbreak of infectious diseases with spleen necrosis as the main symptom has occurred in land ducks in Shandong, Hebei, Henan, Anhui and Jiangsu. The disease mainly causes spleen enlargement, hemorrhage and necrosis in ducks of all ages, resulting in duck breeding The weight of eggs and meat ducks decreases, the feed-to-meat ratio increases, and the pass rate of meat ducks drops significantly, causing serious economic losses to the meat duck and duck breeding industry
However, because the disease is reported for the first time in my country, there is no commercial vaccine and antibody on the market, and the egg yolk antibody used for the emergency prevention and early infection treatment of this new type of duck reovirus has not been reported; moreover, the egg yolk antibody It is obtained by immunizing laying hens, but the immune response of immunized laying hens is difficult to predict, because there are many factors that affect the immune effect, the most important factors are antigen (including dosage and molecular weight), type of adjuvant, and immunization procedure etc. In addition, the yolk contains a large amount of lipids, which brings a lot of inconvenience to use, which also restricts the development and application of the yolk antibody for preventing and treating this novel duck reovirus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Discovery and identification of novel duck reovirus

[0060] 1. Virus isolation:

[0061] (1) The LMH cell line, which is more susceptible to reovirus, was selected as the cell line, and the LMH cell line frozen in the laboratory was resuscitated, and cultured until the cells were in good condition, suitable for virus inoculation for future use;

[0062] (2) Take the spleen of the duck with severe spleen necrosis symptoms submitted for inspection and add sterilized saline at a ratio of 1:5 (W / V) for homogenization, freeze and thaw the homogenate repeatedly 3 times, centrifuge at 6000rpm for 15min, and take The supernatant was used for later use. Double antibodies (penicillin, streptomycin) 8000U / mL were added to the supernatant, and placed at 4°C overnight. Take the supernatant and filter bacteria and other substances with a filter to obtain the original virus mixture, inoculate the mixture into the susceptible cell line LMH for passage, and pass it to 5 ge...

Embodiment 2

[0066] Embodiment 2: the preparation of inactivated vaccine

[0067] (1) Virus propagation procedure: Inoculate 9-day-old healthy SPF chicken embryos with the novel duck reovirus strain isolated in Example 1 at a dose of 0.2ml / piece, discard dead embryos within 24 hours, and collect dead chickens within 24 to 120 hours Embryo allantoic fluid, virus fluid obtained, stored at -20°C.

[0068] (2) Concentration of the virus liquid: Concentrate the harvested virus liquid to 1 / 3 of the original volume with an ultrafiltration concentrator at 2-8°C, and conduct a sterility test according to the appendix of the current "Chinese Veterinary Pharmacopoeia" to ensure aseptic growth , and keep samples for testing the poison price. The virus content per 0.1ml of virus liquid should be ≥10 5.0 ELD 50 , the concentrated allantoic fluid is then inactivated.

[0069] (3) Inactivation: the concentrated virus liquid is introduced into an inactivation tank, and 10% formaldehyde solution is adde...

Embodiment 3

[0077] Example 3: Preparation of novel duck reovirus yolk antibody

[0078] 1. Use inactivated vaccines to immunize laying hens:

[0079]The new reovirus inactivated vaccine prepared in Example 2 is used to immunize laying hens, and the neck of each chicken is subcutaneously injected with 2.0ml of the inactivated vaccine for the first time. After 14 days, the second immunization is carried out, and the neck of each chicken is subcutaneously injected with 2.0ml. Inactivated vaccine, the third immunization was carried out 14 days after the second immunization, and 2.0ml inactivated vaccine was injected subcutaneously in the neck of each chicken, and booster immunization was carried out 14 days after the third immunization, 2.0ml inactivated vaccine was injected subcutaneously in the neck of each chicken, 14 days after the booster immunization, the egg yolk was collected to determine the neutralizing antibody titer of the new reovirus should be ≥1:1024.

[0080] 2. Yolk antibody...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a yolk antibody against duck reovirus, and a preparation method and application of the yolk antibody. The yolk antibody obtained by taking inactivated duck reovirus with a preservation number of CCTCC NO: V201843 as an antigen for preparing a vaccine immunized laying hen and performing extraction and purification on high-immunity egg yolks. The prepared yolk antibody is good in safety and does not cause any local or systemic adverse reactions, also can effectively prevent and / or treat infection of novel duck reovirus, and has good commercial development prospect.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an anti-duck reovirus yolk antibody and its preparation method and application. Background technique [0002] The genome of reovirus is a double-strand segmented RNA, which was designated as Reoviridae by the International Committee for Isolation of Viruses (ICTV) in 1978, including the genus Reovirus, Circovirus and Rotavirus. Symptovirus. Reovirus (Avian reovirus) was isolated from the respiratory tract of chickens for the first time. After infecting susceptible chickens, symptoms such as tendinitis, synovitis and respiratory diseases can be manifested. ARV can infect chickens, turkeys, ducks, geese, parrots, pigeons and other poultry. It can cause viral arthritis and tenosynovitis in chickens. It is also the cause of other diseases, including myocarditis, pericarditis, hydropericardium, hepatitis, and thymus atrophy. , respiratory disease, and growth r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14
CPCA61K2039/505A61P31/14C07K16/02C07K16/10C07K2317/11C07K2317/35
Inventor 唐熠刁有祥王鸿志
Owner SHANDONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com