Primers, kits and applications for controlling wheat leaf senescence

A technology of leaf senescence and wheat, which is applied in the fields of plant genetic engineering and wheat molecular breeding, can solve the problems of being difficult to be used and poor agronomic traits

Active Publication Date: 2021-10-12
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although delayed senescence mutants have been studied in many crops, most of them have poor agronomic traits and are difficult to exploit

Method used

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  • Primers, kits and applications for controlling wheat leaf senescence
  • Primers, kits and applications for controlling wheat leaf senescence
  • Primers, kits and applications for controlling wheat leaf senescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. TaNACS gene cloning and overexpression vector construction

[0056] Using Chinese spring wheat cDNA as template, PCR amplification was carried out with SEQ ID NO.1 as upstream primer and SEQ ID NO.2 as downstream primer. The template concentration was 50ng / ml, and the amplification procedure was as follows:

[0057] 95°C for 3min; 33 cycles of 95°C for 30s, 59°C for 30s, 72°C for 1min; 72°C for 10min.

[0058] The PCR amplified product was connected to the pCambia3301 overexpression vector (ubi-driven overexpression) by infusion recombinase, finally forming the recombinant vector pCambia3301-TaNACS.

[0059] The connection method is:

[0060] (1) Digest the pCambia3301 vector with EcoRI and BamHI, and recover the fragments for later use;

[0061] (2) Establish the ligation system: 1.5 μL of the digested pCambia3301 vector; 2.5 μL of PCR fragments; 1 μL of infusion enzyme:

[0062] (3) Establish reaction conditions: 50°C for 15 minutes, place on ice for 5 minutes; ...

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Abstract

The invention discloses a primer, kit and application for controlling wheat leaf senescence, which belongs to the technical field of plant genetic engineering and wheat molecular breeding, and can solve the problem of poor agronomic traits of crops in delaying many crop senescence mutants. Difficult to exploit. The technical solution includes designing specific upstream and downstream primers based on the wheat gene TaNACS, promoting the senescence of wheat leaves by overexpressing the TaNACS gene, and using RNAi technology to silence the TaNACS gene in wheat to delay the senescence of wheat leaves. The invention uses the wheat TaNACS gene to control the senescence process of wheat leaves, and at the same time, neither the overexpression of the gene nor the gene silencing material affects other agronomic traits of the wheat.

Description

technical field [0001] The invention belongs to the technical fields of plant genetic engineering and wheat molecular breeding, and in particular relates to a primer for controlling wheat leaf senescence, a kit and an application thereof. Background technique [0002] Senescence is the last stage of plant development. It is not only affected by external factors such as temperature, disease, and stress, but also regulated by genes and affected by hormone levels in the body. Leaf senescence is closely related to crop yield and quality. Premature or late senescence of crops is not conducive to completing its normal life cycle, resulting in reduced yield or quality of crops, which seriously affects agronomic traits. Therefore, the discovery and application of leaf senescence regulators is one of the effective ways to achieve high yield and high quality of crops. [0003] Wheat is one of the most important food crops in the world, and improving wheat yield is the goal of many br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 张增林郭永峰李伟高晓明徐萌萌
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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