A free triiodothyronine fT3 detecting kit based on a micro-fluidic chip, and preparation and detection methods thereof

Abstract

A free triiodothyronine fT3 detecting kit based on a micro-fluidic chip, and preparation and detection methods thereof are disclosed. The preparation method of the kit includes (1) coating the micro-fluidic chip, (2) labeling fluorescent microspheres, (3) assembling the micro-fluidic chip, (4) preparing a standardization product, and (5) preparing the free triiodothyronine fT3 kit based on the micro-fluidic chip. The prepared kit performs measurement through a competition process, wherein a time-resolved fluorescent material high in sensitivity is adopted as a marker to perform antigen markingon the fluorescent microspheres, analysis and detection are performed by utilizing immune competition, and a prepared agent has performance reaching the level of a chemiluminescence agent of the sameclass. After an immunoreaction, excessive components are completely removed with a cleaning liquid, the fluorescent microspheres combined through the immunoreaction is detected and read, and a problem that coloration liquids introduced into the chip are not sufficiently reacted or reading after coloration is behind time is avoided.

Description

technical field [0001] The invention belongs to the field of immune detection, and in particular relates to a kit for detecting free triiodothyronine fT3 based on a microfluidic chip and a preparation and detection method. Background technique [0002] Microfluidic chip technology is characterized by a micropipeline network, using micromachining technology to etch a micropipeline network and other functional units on a chip of a few square centimeters in size, thereby preparing a microfluidic chip that includes injection, reaction, separation, and detection An integrated fast, efficient, and low-consumption micro-analysis device. The microfluidic chip has the characteristics of less reagent consumption, short reaction time and high degree of automation in the detection platform. In the current research, the miniaturization of the chip has brought many difficulties to the early sample separation and supporting integration, and there are also many problems in sample fixation ...

AI Technical Summary

Problems solved by technology

[0013] Using other existing technologies or (1) the structure of the chip is too complicated to precisely regulate the reaction, and there is a large pollution in the cleaning steps and liquid conduction; or (2) multiple liquid chambers and solid phase carriers are used (magnetic beads and fluorescent microspheres are used at the same time), the overall design of the reagent is too complicated; or (3) the immunoreaction adopts a heterogeneous reaction, which means that the solid phase carrier reacts with the surface of the solid phase carrier, the reaction is insufficient, and the sensitivity and Reproducibility is limited; or (4) the fluorescence or chemiluminescence reaction with higher sensitivity is not selected, but the turbidimetric reaction or chromatography reaction with lower sensitivity is used, especially when the reaction reagent of the chromatography system is used, the The fundamental problem of incomplete antibody release from chromatography reagent strips and the poor detection accuracy of one-way reactions lead to compromised test results

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