Method for preparing abalone active glycopeptide by subcritical fluid extraction and enzyme membrane coupling reaction and reaction system

Abstract

The invention relates to the field of preparation of glycopeptides, and discloses a method for preparing an abalone active glycopeptide and a reaction system. The method for preparing the abalone active glycopeptide comprises the following steps: (1) subcritical fluid extraction: heating a mixture of abalone viscera and water to 130 to 150 DEG C for 0.1 to 5 hours for performing subcritical fluidextraction to obtain an extraction liquid; (2) deodorization and degreasing: deodorizing and degreasing the extraction liquid by activated adsorption carbon to obtain an impurity removal liquid; (3) enzyme membrane coupling reaction: subjecting the impurity removal liquid to an enzymatic hydrolysis reaction, continuously filtering an enzymatic hydrolysate with an ultrafiltration membrane having amolecular weight cut off of 5-20 kDa in the enzymatic hydrolysis reaction process to obtain a dialysate and a retentate, and the retentate returning to the enzymatic hydrolysis reaction step; (4) desalting treatment. The abalone active glycopeptide can be prepared by the method provided by the invention, the reaction time can be shortened, the obtained glycopeptide is light yellow or brown, the quality is good, the enzyme amount can be reduced, the enzyme recycling rate can be improved, the energy consumption can be reduced, and the production cost can be reduced.

Description

technical field [0001] The invention belongs to the field of glycopeptide preparation, and in particular relates to a method and a reaction system for preparing abalone active glycopeptide through a coupling reaction of subcritical fluid extraction and enzyme membrane. Background technique [0002] Abalone active glycopeptide is a high molecular glycopeptide polymer extracted from the viscera of abalone. At present, the preparation process of abalone active glycopeptides is generally to break the abalone visceral cells through a meat grinder, and use water extraction to dissolve the water-soluble substances in the abalone viscera, and then use compound proteases or various proteolytic enzymes for multiple hydrolysis, and the obtained enzymatic hydrolysis After the substrate is degreased, deodorized and decolorized, it is treated with enzyme inactivation, and then it is filtered through multi-level membranes (such as ceramic membrane filtration, ultrafiltration membrane, nano...

AI Technical Summary

Problems solved by technology

[0003] (1) Long-time boiling water extraction accelerates the occurrence of non-enzymatic browning, causing the amino acids, polypeptides, oligosaccharides, etc. in the extract to undergo Maillard reactions, resulting in serious loss of effective active ingredients, and the extract is dark brown , deep in color, the final product is brown in color and has a heavy burnt smell, which affects the flavor quality of the final product;

[0004] (2) Enzymatic hydrolysis: The enzymatic hydrolysis takes a long time, and the processing auxiliary materials such as enzymes are seriously lost, and cannot be reused;

[0005] (3) Need to kill enzymes: heating to kill enzymes will increase energy consumption;

[0006] (4) Membrane separation: Long membrane separation time will increase energy consumption

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