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CYP2C19 gene detecting method and detecting kit

A CYP2C19 and gene detection technology, applied in the field of biomedicine, can solve problems such as liver damage

Inactive Publication Date: 2018-12-11
为康(苏州)基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Sodium valproate and magnesium valproate are currently the first choice drugs for the treatment of generalized or partial epilepsy, but the metabolites of valproic acid have certain hepatotoxicity and damage the liver

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  • CYP2C19 gene detecting method and detecting kit
  • CYP2C19 gene detecting method and detecting kit
  • CYP2C19 gene detecting method and detecting kit

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Embodiment Construction

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] It should be noted that when a component is said to be "fixed" to another component, it can be directly on the other component or there can also be an intervening component. When a component is said to be "connected" to another component, it may be directly connected to the other component or there may be intervening components at the same time. When a component is said to be "set on" another component, it may be set directly on the other component or t...

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Abstract

The invention discloses a CYP2C19 gene detecting method which comprises the following steps: extracting sample DNA; designing a specific primer for amplifying a CYP2C19 gene segment; utilizing singletube multi-PCR amplification reaction to obtain a target segment; designing a single base extension primer for the CYP2C19 gene segment; extending the single base of the CYP2C19 gene segment; and analyzing a nucleic acid mass spectrum for measuring a target DNA sequence. The CYP2C19 gene detecting method can effectively perform CYP2C19 gene polymorphic screening on a patient, and lays clinical andscientific research basis for performing personalized accurate diagnosis and treatment. The invention further relates to a CYP2C19 gene detecting kit.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a CYP2C19 gene detection method and a detection kit. Background technique [0002] Cytochrome oxidase P450 2C19 (CYP2C19), also known as S-mephenytoin hydroxylase, is a member of cytochrome P450. There are obvious individual differences in its activity, which affects the metabolism of many clinical drugs, and the drug treatment of different individuals The effects and adverse reactions and the toxicity of the drug have an important impact. In 1993, Wrighton et al. proved that S-mephenytoin was oxidized to 4'-hydroxymephenytoin by the hydroxylase encoded and expressed by CYP2C19 in human liver, and the activity of CYP2C19 enzyme could be judged according to the level of mephenytoin hydroxylation metabolism . In 1994, Morais found that two polymorphisms on CYP2C19: ml(*2rs4244285), m2(*3rs4986893) could cause weak metabolism of S-mephenytoin, and these two polymorphisms could explain &...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2537/143C12Q2547/101C12Q2533/101C12Q2565/627
Inventor 王文忠田军龙胡军张为陈苏平
Owner 为康(苏州)基因科技有限公司
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