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A strain of Pseudomonas putida bc10 and its application in the control of bacterial soft rot in crops

A Pseudomonas putida, bacterial technology, applied to Pseudomonas putida BC10 and its application in preventing and treating bacterial soft rot of crops, can solve problems such as toxicity, bacterial resistance, water resources pollution, etc. Achieve good inhibitory effect, good control effect, and reduce the occurrence of bacterial soft rot of crops

Active Publication Date: 2020-02-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical agents have certain toxicity to humans and animals, and can also cause problems such as bacterial resistance and water pollution.

Method used

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  • A strain of Pseudomonas putida bc10 and its application in the control of bacterial soft rot in crops
  • A strain of Pseudomonas putida bc10 and its application in the control of bacterial soft rot in crops
  • A strain of Pseudomonas putida bc10 and its application in the control of bacterial soft rot in crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and screening of Pseudomonas putida BC10

[0036] 1. Strain isolation

[0037] (1) Sample collection: In the rhizosphere soil of cabbage in Dalingxia Village, Yashan Village Committee, Quzhang Township, Hepu Township, Guangxi, soil samples below 5 cm of the soil surface were excavated and stored in bags, and brought back to the laboratory for strain isolation.

[0038] (2) Isolation of bacterial strains: Soil samples were isolated by dilution coating plate method. Specifically, after the collected soil samples were thoroughly mixed by hand, 4.0 g was weighed, soaked in 20 mL of sterile water and shaken, and then cultured on a shaker at 28 °C and 180 rpm for 20 min for gradient dilution. Take the dilution factor as 10 5 ~10 9 100 µL of the diluted solution was spread on the LB plate, air-dried at room temperature, sealed, and incubated upside down in a 28°C incubator for 24 h. Pick a single colony grown on the LB plate, label it, place it in a 2 m...

Embodiment 2

[0041] Example 2 Identification of Pseudomonas putida BC10

[0042] 1. Morphological and physiological and biochemical identification

[0043] The colonies of BC10 on LB medium are round, milky white to yellowish, with smooth and neat edges, slightly raised in the middle, opaque, Gram stain negative, can liquefy gelatin, produce acid and catalase, but not amylase, Fructose can be used, trehalose can be used in a small amount, and galactose, mannitol, glucose and sucrose cannot be used.

[0044] 2. Molecular identification

[0045] In order to clarify the taxonomic status of the strain BC10 obtained in Example 1, the method of multilocus sequence analysis (MLSA) combined with phylogenetic analysis (Phylogenetic analysis) was used to identify the BC10 strain.

[0046] 16S rDNA sequence analysis is a molecular biological identification method for bacterial identification. The present invention analyzes the 16S rDNA sequence and combines the conservative housekeeping gene gyrB ,...

Embodiment 3

[0049] Example 3 Determination of Pseudomonas putida BC10 bacteriostatic spectrum

[0050] 1. Determination of antibacterial spectrum

[0051] In order to better study the biocontrol potential of the bacterial strain BC10 of the present invention, the antibacterial spectrum of the bacterial strain was studied. The specific operation is as follows:

[0052] (1) Explore whether BC10 has the ability to inhibit the growth of other pathogenic bacteria. Use the method identical with embodiment 1 bacterial strain screening, change pathogenic bacterium MS2 into the equal amount of rice base rot pathogen EC1, banana bacterial soft rot pathogen MS2 and MS3, bacterial wilt pathogen EP1 (first Layer 15 mL of LB into 15 mL of TTC), rice bacterial blight Xoo, rice soft rot fungus SC7 and peach rot fungus PP1, and the rest of the steps remain unchanged.

[0053] (2) To explore whether BC10 has the ability to inhibit the growth of pathogenic fungi. The fungi tested included capsicum anthr...

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Abstract

The invention discloses a strain of Pseudomonas putida ( Pseudomonas putida ) BC10 and its application in the control of bacterial soft rot in crops. The strain was deposited in the Guangdong Microbial Culture Collection Center on July 10, 2018, with the preservation number GDMCC NO.60411. The strain has a significant inhibitory effect on a variety of bacterial pathogens, especially on rice bacterial root rot, banana bacterial soft rot and Clivia soft rot, and can reduce crop bacterial soft rot The occurrence of the disease, the control effect is better in the pot test, and the strain BC10 itself has no pathogenicity, indicating that the strain can be used to develop biopesticides for bacterial diseases of crops.

Description

technical field [0001] The invention belongs to the technical field of plant disease biocontrol. Specifically, it relates to a strain of Pseudomonas putida BC10 and its application in controlling bacterial soft rot of crops. Background technique [0002] Dixonella ( Dickeya ) is one of the world's top ten important plant pathogenic bacteria, and is listed as my country's No. 302 quarantine pest, which can cause severe bacterial soft rot in crops and flowers. in, D. dadantii , D. zeae with D. solani often cause major crop diseases, especially from D. zeae Rice bacterial base rot and banana bacterial soft rot caused by D. solani Bacterial soft rot of potatoes caused by it has been expanding and aggravating in recent years. [0003] D. cornella ( D. zeae ) has a wide range of hosts. In addition to infecting monocotyledonous plants, it can also infect dicotyledonous plants at the same time. Its host range includes rice, corn, sugarcane, banana, brachiaria, iris, sh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P1/00C12R1/40
CPCA01N63/00C12N1/20C12N1/205C12R2001/40
Inventor 周佳暖陈霞李洁玲胡明张炼辉徐领会
Owner SOUTH CHINA AGRI UNIV
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