A method for detecting fructo-oligosaccharides and raffinose in infant milk powder
A technology of fructooligosaccharides and raffinose, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the simultaneous detection method has not been reported, the composition information cannot be obtained, and the operation process is complicated.
Active Publication Date: 2021-04-27
SHANDONG INST FOR FOOD & DRUG CONTROL
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Problems solved by technology
However, the content of fructooligosaccharides in food is determined by indirect method, and the operation process of this method is complicated, only the total amount of oligosaccharides can be obtained, and the specific component information cannot be obtained.
[0006] At present, there is no report on the simultaneous detection of fructo-oligosaccharides and raffinose in infant formula milk powder
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Embodiment 1
[0050] 1. Instruments and materials
[0051] Waters H-CLASS ultra-high performance liquid chromatography equipped with ACQUITY UPLC ELS detector (Waters, USA); chromatographic column: Click XIon chromatographic column (150 mm×3.0 mm i.d, 3 µm).
[0052] The standard substances of kestose, kestose, and kestose pentaose were Japanese Wako standard substance set, with a purity of 99.9%. Raffinose was purchased from Sigma, with a purity of ≥99.5%, and stored at 4°C; acetonitrile (chromatographically pure, Fisher, USA); water is ultrapure water; nitrogen (>99.999%); C18 adsorbent (Agilent, USA). Organic microporous membrane (0.22 µm, purchased from Shanghai Anpu Scientific Instrument Co., Ltd.).
[0053] 2. Pretreatment
[0054] 2.1 Standard stock solution
[0055] Accurately weigh 100mg (accurate to 0.1mg) of each standard substance of fructo-oligosaccharides (kestose, kestosetetose, and kestosepentaose) and raffinose in a 10 mL volumetric flask, dissolve in water and dilute to...
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Abstract
The invention discloses a detection method of fructooligosaccharide and raffinose in infant milk powder, which comprises the following steps: (1) pretreatment; (2) configuration of standard solution; (3) obtaining liquid chromatogram, regression Equation; (4) Qualitative analysis; (5) Quantitative calculation, using external standard method to quantify: according to the area of the chromatographic peak in the sample chromatogram, adopt the regression equation of step (3), calculate and get kestose, cane fruit four sugar, fructopentose or / and raffinose; in this study, a new type of hydrophilic separation material was selected, combined with high performance liquid chromatography, a simple, rapid and accurate detection of oligomerization in infant formula milk powder was developed. A qualitative and quantitative method for the simultaneous detection of fructose and raffinose.
Description
technical field [0001] The invention relates to a method for detecting fructooligosaccharides and raffinose in infant milk powder, and belongs to the technical field of food detection. Background technique [0002] Fructo oligosaccharide (FOS) is an oligosaccharide composed of sucrose and 1~3 fructose through β-(2,1) glycosidic bonds, including kestose (GF2), kestetose ( GF3), fructopentose (GF4) and other ingredients. FOS has the function of promoting the growth of intestinal bifidobacteria and regulating the balance of intestinal flora. Raffinose is a functional oligosaccharide composed of galactose, fructose and glucose, which also has a strong effect on the proliferation of bifidobacteria. Because the baby's intestinal development function is immature, it is prone to intestinal flora imbalance, and is infected by external viruses or bacteria, causing diarrhea and other diseases. Utilizing fructo-oligosaccharide and raffinose to regulate the function of intestinal flor...
Claims
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IPC IPC(8): G01N30/02G01N30/74
CPCG01N30/02G01N30/74
Inventor 张艳侠刘艳明薛霞徐向军于文江郭志谋闫竞宇别梅孙立臻
Owner SHANDONG INST FOR FOOD & DRUG CONTROL