Application of naphthoquinone compound in preparing drug for treating or preventing pancreatic cancers
A compound, pancreatic cancer technology, applied in drug combination, digestive system, antitumor drugs, etc., can solve problems such as pancreatic cancer cell cycle arrest
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Embodiment 1
[0063] Example 1 plumbagin inhibits the differentiation of PBMCs to MDSCs in vitro
[0064] 1.1 Objects and methods
[0065] 1.1.1 Reagent preparation
[0066] (1) Preparation of 0.0lM PBS (PH 7.2‐7.4):
[0067] Weigh Na with a balance 2 HPO 4 1.54g, KH 2 PO 4 0.2g, NaCl 8.0g, KCl 0.2g, add the reagents into 800mL double-distilled water, stir until dissolved, then adjust the volume to 1L, adjust the pH value to 7.2-7.4. Autoclave for future use, and store at 4°C for future use.
[0068] (2) Preparation of antibody diluent:
[0069] Weigh 0.2mg BSA with a balance, pipette 1mL 10% NaN 3 And add 80mL of PBS, stir until dissolved, and then dilute to 100mL. Store in a 4°C refrigerator for later use.
[0070] (3) Staining buffer (Staining buffer, SB) preparation:
[0071] Pipette 1mL of FBS and 10% NaN respectively 3 And add 80mL of PBS, stir and mix well and then dilute to 100mL. Store in a 4°C refrigerator for later use.
[0072] (4) Preparation of complete medium ...
Embodiment 2
[0202] Example 2 plumbagin inhibits the growth of pancreatic cancer in mice
[0203] 2.1 Objects and methods
[0204] 2.1.1 Reagent preparation
[0205] (1) Dissolving plumbagin
[0206] Plumbagin was purchased from Bailingwei Technology Co., Ltd. and stored at room temperature.
[0207] Preparation of plumbagin stock solution: Weigh 10 mg plumbagin powder and dissolve it in 1062.8 μL dimethyl sulfoxide (DMSO), vortex until completely dissolved. tube, stored at -20°C.
[0208] (2) Preparation of 10% bovine serum albumin (10% BSA):
[0209] Use a balance to weigh 1g of BSA and add it to 10mL of PBS, and vortex until it is completely dissolved. 10% bovine serum albumin (10% BSA) needs to be stored in a refrigerator at 4°C.
[0210] (3) Preparation of tumor tissue digestion solution (50×):
[0211] Weigh 15 mg of collagenase IV, 15 mg of deoxyribonucleotidase I and 15 mg of hyaluronidase into 6 mL of PBS, vortex fully until completely dissolved, filter with a 0.22 μm filte...
Embodiment 3
[0283] Example 3 Research on the Mechanism of Plumbagin Inhibiting the Differentiation of MDSCs
[0284] 3.1 Objects and methods
[0285] 3.1.2 Reagent preparation
[0286] (1) Preparation of 0.0l M PBS (PH 7.2‐7.4):
[0287] Weigh Na with a balance 2 HPO 4 1.54g, KH 2 PO 4 0.2g, NaCl 8.0g, KCl 0.2g, add the reagents into 800mL double-distilled water, stir until dissolved, then adjust the volume to 1L, adjust the pH value to 7.2-7.4. Autoclave for future use, and store at 4°C for future use.
[0288] (2) Preparation of Running buffer:
[0289] Weigh 14.4g of glycine, 3.02g of Tris-base, and 1.0g of SDS with a balance, add the reagents into 800mL of double-distilled water, stir until dissolved, and then dilute to 1L.
[0290] (3) Preparation of Trans buffer:
[0291] Weigh 14.4g of glycine, 3.02g of Tris-base, and 1.0g of SDS with a balance, add the reagents into 700mL of double-distilled water, stir until dissolved, add 200mL of methanol, stir well and then dilute t...
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