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A kind of anti-heavy metal plant growth-promoting bacterial strain and application thereof

A technology that resists heavy metals and promotes plant growth. It is applied in the direction of plant growth regulators, plant growth regulators, and applications. It can solve the problems of reduced utilization and achieve the effects of reducing root accumulation, reducing accumulation, and promoting growth.

Active Publication Date: 2020-08-14
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year

Method used

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  • A kind of anti-heavy metal plant growth-promoting bacterial strain and application thereof
  • A kind of anti-heavy metal plant growth-promoting bacterial strain and application thereof
  • A kind of anti-heavy metal plant growth-promoting bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 : Isolation and Identification of Acinetobacter soli BHG-02

[0036] 1.1 Isolation of Acinetobacter soli BHG-02

[0037] The isolation of Acinetobacter soli (Acinetobacter soli) BHG-02 includes 3 steps of sampling, enrichment, primary screening, and secondary screening, as follows:

[0038] 1.1.1 Sampling

[0039] Sampling was taken from the soil of Jinwan Mangrove Reserve in Beihai City, Guangxi Zhuang Autonomous Region, put into a clean sampling bag (bottle), marked, and stored in a 4°C refrigerator for later use.

[0040] 1.1.2 Enrichment and primary screening

[0041] Inoculate 1 g of the collected sample into 100 mL of [CuSO 4 ]=3mM LB medium, 37°C, 200rpm, shaking culture for 24h. Then take 1 mL of the supernatant, spread it on the LB plate with [NaCl]=800mM by ten-fold gradient dilution method, incubate for 48 hours at 37°C, observe whether there are colonies, and transfer the grown colonies to a new medium containing the corresponding concentrati...

Embodiment 2

[0057] Example 2 : Acinetobacter soli (Acinetobacter soli) BHG-02 growth-promoting effect of growth-promoting bacteria under aluminum stress

[0058] 2.1 The tested strains promote the growth of plant seeds in hydroponic culture

[0059] ①Put the filter paper in the petri dish, cover the bottom of the dish as much as possible, sterilize with damp heat at 121°C for 20 minutes, dry at 70°C, and set aside.

[0060] ②Preparation of bacterial suspension: inoculate the strain in LB medium, culture at 37°C, 200rpm for 18 hours, collect the bacterial cells by centrifugation, suspend with distilled water, add distilled water to OD 600 =0.9, the bacterial suspension is about 10 9 CFU / mL.

[0061] ③ Seed surface disinfection: pick out plump seeds, wash three times with sterile distilled water, wash twice with 75% alcohol, soak in 75% alcohol for 2 minutes, wash three times with sterile distilled water, and set aside. The bacterial suspension was serially diluted, and the dilution co...

Embodiment 3

[0066] Example 3 : Determination of the types and amounts of organic acids produced by Acinetobacter soli BHG-02

[0067] The BHG-02 strain was continuously fermented in liquid NBRIP liquid medium at 32°C and 200rmp / min for 5 days, and then the organic acids in the fermentation liquid were analyzed by high performance liquid chromatography. An Aminex HPX-87H organic acid and alcohol analytical column (BIO-RAD) with a length of 250 mm was used. Conditions: column temperature is 65°C, mobile phase is 5mmol L -1 Sulfuric acid (prepared with ultrapure water, degassed by ultrasonic wave for 15min), the flow rate is 0.5mL min -1 , detected by a differential refractive index detector, and the injection volume was 0.02 mL.

[0068] The analysis results show that BHG-02 can effectively use the glucose in the culture medium to convert it into citric acid, and then dissolve the calcium phosphate in the culture medium, dissolve the metal calcium chelate, and release the available phos...

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Abstract

The invention belongs to the technical field of plant growth-promoting bacteria, and specifically relates to a plant growth-promoting bacterial strain resistant to heavy metals and an application thereof. A heavy metal-resistant plant growth-promoting bacterial strain, which is named Acinetobacter soli (Acinetobacter soli) BHG-02 in taxonomy, was preserved in the China Center for Type Culture Collection on October 13, 2014, and the preservation number is CCTCC NO: M2014471 . The anti-heavy metal growth-promoting plant strain BHG-02 of the present invention is first screened and isolated from the soil of the Jinwan Mangrove Reserve in Beihai City, Guangxi Zhuang Autonomous Region. It can secrete organic acids, dissolve and utilize calcium phosphate in the culture medium, At the same time, it has the characteristics of ACC deaminase activity, growth hormone IAA, urease activity, etc., dissolves heavy metals in the soil, relieves heavy metal stress of plants, improves soil nutrient status, and thus plays a role in promoting plant growth.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and specifically relates to a plant growth-promoting bacterial strain resistant to heavy metals and an application thereof. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production. However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year. Residues of pesticides and chemical fertilizers have released a large amount of harmful substances to the environment, polluting soil, water sources and food, and posing a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food has become a top priority. Microb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N43/38C05F11/08A01P21/00C12R1/01
CPCA01N43/38C05F11/08C12N1/205C12R2001/01
Inventor 李有志樊宪伟陈登峰
Owner GUANGXI UNIV
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