A plant growth-promoting bacterial strain ng-33 for decomposing inorganic phosphorus and its application

A technology of NG-33, inorganic phosphorus, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as lower utilization rate, and achieve the effect of saving phosphorus fertilizer, promoting plant growth, and improving soil

Active Publication Date: 2018-11-20
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year

Method used

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  • A plant growth-promoting bacterial strain ng-33 for decomposing inorganic phosphorus and its application
  • A plant growth-promoting bacterial strain ng-33 for decomposing inorganic phosphorus and its application
  • A plant growth-promoting bacterial strain ng-33 for decomposing inorganic phosphorus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 : Isolation and identification of Enterobacter cloacae NG-33 (Enterobacter cloacae)

[0034] 1.1 Isolation of Enterobacter cloacae NG-33 (Enterobacter cloacae)

[0035] The isolation of Enterobacter cloacae NG-33 (Enterobacter cloacae) includes 3 steps of sampling, enrichment, primary screening, and secondary screening, as follows:

[0036] 1.1.1 Sampling

[0037] Samples were taken from the soil of Nonggang National Nature Reserve in Guangxi Zhuang Autonomous Region, put into clean sampling bags (bottles), marked, and stored in a 4°C refrigerator for later use.

[0038] 1.1.2 Enrichment and primary screening

[0039] Weigh 10 g of the collected sample and pour it into 100 mL of Luria-Bertani (LB) liquid medium containing 800 mM, and culture with shaking at 37° C. at 200 rpm for 24 hours. Then take 1mL of the bacterial suspension and inoculate it into a Erlenmeyer flask equipped with NBRIP liquid medium. Multiple shake flasks are tested in parallel, with ...

Embodiment 2

[0053] Example 2 : Determination of Soluble Phosphorus Content in Shake Flask Culture of Enterobacter cloacae NG-33 (Enterobacter cloacae)

[0054] 2.1 Configuration of molybdenum antimony antichromogenic solution

[0055] ① Take antimony potassium tartrate [K(SbO)C 4 h 4 o 6 ] 0.5g, dissolved in 100mL water.

[0056] ② Weigh ammonium molybdate [(NH 4 ) 6 Mo 7 o 24 4H 2 O] 10g, dissolved in 450mL water, slowly added 153mL concentrated H 2 SO 4 , while adding and stirring.

[0057] ③Then add the above ① solution to ② solution, and finally add water to 1L. Shake well and store in a brown bottle. This is a mixture of molybdenum and antimony.

[0058] ④Before use, weigh L-ascorbic acid (C 6 h 8 o 5 , chemically pure) 1.5g, dissolved in 100mL molybdenum-antimony mixture, and mixed evenly, this is the molybdenum-antimony antireagent. The validity period is 24 hours, and the validity period is longer if stored in the refrigerator.

[0059] The tested strains were c...

Embodiment 3

[0061] Example 3 : Determination of the types and amounts of organic acids produced by Enterobacter cloacae NG-33 (Enterobacter cloacae)

[0062] The NG-33 strain was continuously fermented in liquid NBRIP liquid medium at 37°C and 180rmp / min for 5 days, and the culture liquid was measured at the same time every day, and then the organic acids in the fermentation liquid were analyzed by ion chromatography. Take 1 mL of fermentation broth and place it in a 2 mL plastic centrifuge tube, centrifuge at 12,000 r / min for 5 min, take the supernatant, dilute 10 times with ultrapure water, and filter the diluted solution through a 0.23 μm filter membrane to remove micron-sized suspended matter. Use ICS-5000 ion chromatography device (Thmero Company, USA), adopt IonPacAS11 anion exchange column, IC-RP guard column, analytical column (250mm×4mm), guard column (50mm×4mm). Gradient elution was adopted, the flow rate was 1 mL / min, the column temperature was 30°C, and the injection volume ...

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Abstract

The invention belongs to the technical field of plant growth-promoting bacteria, and specifically relates to a plant growth-promoting bacterial strain NG‑33 for decomposing inorganic phosphorus and its application. A plant growth-promoting bacterial strain NG-33 that decomposes inorganic phosphorus, and its taxonomic name is Enterobacter cloacae NG-33 (Enterobacter cloacae), which was preserved in the China Type Culture Collection Center on January 21, 2016, and the preservation number is CCTCC M 2016056. The plant growth-promoting bacterial strain NG-33 of the present invention is screened and isolated from the soil of Nonggang National Nature Reserve in Guangxi Zhuang Autonomous Region for the first time, and can produce high-yield gluconic acid and acetic acid, dissolve insoluble inorganic phosphorus and increase the content of soluble phosphorus in the soil , relieve the phosphorus deficiency stress of plants, improve soil conditions, and thus play a role in promoting plant growth.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and specifically relates to a plant growth-promoting bacterial strain NG-33 for decomposing inorganic phosphorus and its application. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production. However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year. Residues of pesticides and chemical fertilizers have released a large amount of harmful substances to the environment, polluting soil, water sources and food, and posing a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food has become a top priorit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 樊宪伟陈鑫鑫李有志
Owner GUANGXI UNIV
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