Kit for in vitro culture of mouse sperm for improving blastocyst rate and using method thereof

A technology of in vitro culture and sperm culture medium, which is applied in the field of in vitro culture kits, can solve the problems of insufficient improvement of sperm motility and the need to increase the ratio, and achieve the effect of improving sperm motility and blastocyst rate and reducing preparation costs

Active Publication Date: 2022-02-11
SUZHOU MUNICIPAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But two outstanding problems are: 1. The improvement of sperm motility is not enough; 2. The ratio of fertilized zygotes to develop into blastocyst stage (blastocyst rate) needs to be improved

Method used

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  • Kit for in vitro culture of mouse sperm for improving blastocyst rate and using method thereof
  • Kit for in vitro culture of mouse sperm for improving blastocyst rate and using method thereof

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with accompanying drawing.

[0026] Mouse sperm in vitro culture kit to improve blastocyst rate, including box body, 10mL of mHTF (Modified Human Tubal Fluid, modified human tubal fluid), 100mg of BSA (Bovine Serum Albumin), 5ug of ERO1L recombinant protein And 10ug of DPEP2 recombinant protein. mHTF, BSA, ERO1L recombinant protein, and DPEP2 recombinant protein are respectively stored in four disconnected cavities in the box body.

[0027] The ERO1L recombinant protein is an endoplasmic reticulumoxidoreductase 1like protein (ie, endoplasmic reticulumoxidoreductase 1like), and its gene ID is 50527 in the DNA sequence database established by the National Center for Biotechnology Information (NCBI, National Center for Biotechnology Information).

[0028] The DPEP2 recombinant protein is dipeptidase-2 (ie, dipeptidase 2), and its gene ID in the DNA sequence database established by the National Center for...

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Abstract

The invention discloses a mouse sperm in vitro culture kit and a mouse fertilization method for improving the blastocyst rate. Currently, the blastocyst rate of mouse in vitro fertilization is low, which is not conducive to the development of assisted reproductive technology research based on mouse embryos. The mouse sperm in vitro culture kit for improving blastocyst rate of the present invention comprises a milliliter of mHTF, b milligram of BSA, c microgram of ERO1L recombinant protein and d microgram of DPEP2 recombinant protein. a:b:c:d=2:20:1:2. The ERO1L recombinant protein is an endoplasmic reticulum oxidoreductase 1-like protein, and its gene ID in the DNA sequence database is 50527. The DPEP2 recombinant protein is dipeptidase-2, and its gene ID in the DNA sequence database is 319446. In the present invention, ERO1L recombinant protein and DPEP2 recombinant protein are added in the kit, so that the present invention can significantly improve sperm motility and blastocyst rate.

Description

technical field [0001] The invention belongs to the technical field of culture kits, and in particular relates to an in vitro culture kit for improving mouse sperm motility and blastocyst rate and a use method thereof. Background technique [0002] Fertilization is the process in which sperm and egg combine to form a fertilized egg (zygote), which marks the beginning of a new life. In this process, the sperm and egg carriers come from the genetic material of the father and mother respectively, and the quality of the sperm and eggs determines the success or failure of the early embryo development. Currently, much of what is known about the mysterious biological event of fertilization comes from research on in vitro fertilization. However, so far, human understanding of fertilization has not been exhausted, and it is still necessary to rely on in vitro fertilization technology to further study the mechanism of fertilization. [0003] Mouse models are currently an important m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073C12N5/076
CPCC12N5/0604C12N5/061C12N2501/734C12N2501/71C12N2501/998
Inventor 郑波于骏李红吴亦波沈聪陈霞杨慎敏
Owner SUZHOU MUNICIPAL HOSPITAL
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