35results about How to "No toxic effects" patented technology

The invention discloses an RNA with little interference for inhibiting the expression of an iron regulating element regulatory protein (HJV). The RNA with little interference is in reverse complementation with the iron regulating element regulatory protein gene mRNA, and the length of the RNA is 19-27bp. The invention further discloses a DNA for coding the corresponding shRNA of the RNA with little interference and a recombined slow virus capable of expressing the shRNA. The RNA with little interference, the DNA capable of coding the shRNA and the recombined slow virus can effectively inhibit the expression of the iron regulating element regulatory protein in vivo, improve the level of the iron regulating element and reduce the level of serum iron in vivo, thereby reaching the aim of curing diseases related to iron metabolism.

The invention discloses a method for depositing a simvastatin drug-containing hydroxyapatite coating on the surface of pure titanium by utilizing an electrochemical deposition process. The method comprises the following steps: polishing the surface of a pure titanium implant, sand-blasting, acid-etching, and depositing by utilizing an electrochemical deposition station, a simple apparatus and cheap materials to make the simvastatin drug-containing hydroxyapatite coating on the surface of the implant. The method has the advantages of simple preparation process, simple apparatus, low making cost, high making efficiency and the like, and the obtained coating has good biocompatibility and osteogenic cell proliferation differentiation promotion ability. A platform for making the drug-containing coating on the surface of pure titanium is established.

The invention discloses environment-friendly bionic ocean corrosion resisting paint. The environment-friendly bionic ocean corrosion resisting paint is characterized by being prepared from, by weight, 20-25 parts of fluorinated acrylic ester, 22-24 parts of epoxy phenolic resin, 12-15 parts of silicone rubber, 10-12 parts of silicon-aluminum-barium-calcium alloy powder, 3-4 parts of titanium dioxide, 2-4 parts of biological preservative, 3-4 parts of adhesive force thickener, 5-7 parts of filler and 24-28 parts of solvent. Friction coefficient can be reduced effectively, and hydrophobicity can be improved; through the synergistic effect of the filler and the adhesive force thickener, contamination release performance is improved, so that adhesion of attachments does not occur easily or is not firm; the biological preservative has an effective adhesion inhibition effect and has a quite high mussel adhesion preventing capacity; P-cinnamic acid sulfate has a strong marine bacteria and barnacle adhesion inhibition effect, is purely natural and free of pollution, and has a good environment protection effect; the corrosion resisting paint can be sprayed or brushed, and a coating is resistant to corrosion and suitable for severe marine climate.

The invention discloses a marine antifouling nano paint, which is prepared from the following raw materials: epoxy phenolic resin, titanium dioxide, a nano biological antifouling agent, filler, an acetic acid, and glyceryl tristearate. Capsaicin in the nano-scale biological antifouling agent has a good approach-avoidance effect on marine organisms; tannic acid has an adhesion inhibition effect on barnacle and also has a paralysis function on the beetle; shogaol has strong ability of preventing adhesive ability of mussel. P-cinnamic acid sulfate has a strong adhesion inhibition effect on marine bacteria and barnacle, homarine can prevent adhesion of marine benthic diatom and barnacle, and diterpenes formamide has an effect of inhibiting the barnacle and shellfish larvae. The paint disclosed by the invention comprises a nano-scale pure natural biological antifouling agent, has the approach-avoidance effect on marine organisms, and destroys the adhesive ability of the marine organisms, so as to achieve the target of preventing and treating pollution. The marine antifouling nano paint does not generate any deleterious effect on the marine environment, is free of an injury to the marine organisms, and is purely natural and harmless.

The invention relates to a microfluidic device and a screening method for tumor cell screening based on droplet storage, belonging to the field of microfluidic technology. The present invention includes a glass substrate and a PDMS chip fixed thereon, wherein the PDMS material constitutes the entire chip, and a microchannel structure is made by microfabrication technology, a droplet is generated through a cross-shaped structure to wrap a red blood cell or a tumor cell, and then passed through the liquid Droplet register passive valve, due to the difference in size and softness between red blood cells and tumor cells, the number of droplets that wrap red blood cells and those that wrap tumor cells pass through the droplet register is different, and the number of droplets that pass through the droplet register is detected by laser , so as to detect whether there are tumor cells, and realize high-sensitivity real-time detection with a very small sample amount. The invention realizes the integration of fluid sampling, driving, manipulation, transportation and detection and analysis on the microfluidic chip, and realizes controllable liquid flow, transportation of micro and trace substances and efficient detection.

The invention relates to a capacitance type MEMS (micro-electro-mechanical system) gas sensor. The capacitance type MEMS gas sensor is particularly suitable for coal mine safety monitoring as well asdetection on methane gas in the air in production and living, can be applied to detection on the gases such as helium, carbon dioxide and argon in special environments, and comprises a thermal actuator and two comb capacitors, wherein the thermal actuator comprises a plurality pairs of silicon beams, a micro-spindle and an anchor area; and each group of comb capacitor comprises a movable comb, onegroup of folding structure spring, a fixed comb and an anchor area. The sensor can detect the concentration of the methane gas, adopts thermal deformation of the silicon beams to drive the capacitance change of the comb capacitors according to the principle that the resistance of a semiconductor material is changed along with the change of the methane content of the peripheral gas under a certaincurrent, and measures the methane concentration of the air according to the capacitance change. The capacitance type MEMS gas sensor has the following advantages of simple structure, high adaptability, no poisoning influence, no abrasion and no inertness due to capacitance type measurement, high temperature and precision stability, high electromagnetic interference resistance and high dynamic response speed.

The invention provides fusion protein 210 and applications thereof in virus replication optimization. The fusion protein 210 is a truncated part of P60 protein, preserves a function for promoting replication of a plurality of viruses of the P60, and does not generate toxicity on cells in an effective concentration range. The fusion protein 210 can increase the virus titer by two orders of magnitudes in a wide-spectrum range (0.0001-0.1 MOI), and therefore virus identification and detection can be facilitated, rhabdovirus VSV, flaviviridae HCV, parvoviridae EV71, and the like can be detected, and time for detection is shortened by 50% or less. The fusion protein 210 obtained by a prokaryotic expression system is high in expression efficiency and easy to purify, the one-step purification efficiency can be 85% or above, and the protein final concentration can be 3 mg / mL. Accordingly, through directly adding the fusion protein 210 into a virus culture medium, a plurality of virus isolate strains can be detected under low titer conditions, and operation is simple, convenient and rapid. The fusion protein 210 has important influences and significance in the fields of production, learning, researching, and the like of the virology.

The invention provides fusion protein 210 and applications thereof in virus replication optimization. The fusion protein 210 is a truncated part of P60 protein, preserves a function for promoting replication of a plurality of viruses of the P60, and does not generate toxicity on cells in an effective concentration range. The fusion protein 210 can increase the virus titer by two orders of magnitudes in a wide-spectrum range (0.0001-0.1 MOI), and therefore virus identification and detection can be facilitated, rhabdovirus VSV, flaviviridae HCV, parvoviridae EV71, and the like can be detected, and time for detection is shortened by 50% or less. The fusion protein 210 obtained by a prokaryotic expression system is high in expression efficiency and easy to purify, the one-step purification efficiency can be 85% or above, and the protein final concentration can be 3 mg / mL. Accordingly, through directly adding the fusion protein 210 into a virus culture medium, a plurality of virus isolate strains can be detected under low titer conditions, and operation is simple, convenient and rapid. The fusion protein 210 has important influences and significance in the fields of production, learning, researching, and the like of the virology.

The invention relates to a bone scaffold which is used for minimally invasive treatment of cancellous bone fractures of human beings and other animals, such as vertebral compression fracture, calcaneal fracture, tibial plateau collapse fracture and the like. The bone scaffold comprises a scaffold component I and a scaffold component II, wherein a dovetail tenon or a dovetail mortise on the inner side surface of the scaffold component II can slide, are completely inserted into the paired dovetail mortise or the dovetail tenon on the inner side surface of the scaffold component I, and are locked by a locking device, thereby completing the tenon-mortise joint and forming a whole; and the bone scaffold can play the roles of resetting, supporting and fixing in cancellous bone. An intermediate scaffold component can be arranged between the scaffold component I and the scaffold component II, the dovetail tenon or the dovetail mortise on the upper and the lower surface of the intermediate scaffold component can slide simultaneously, are completely inserted into the paired dovetail mortise or the dovetail tenon on the inner side surfaces of the scaffold component I and the scaffold component II, and are locked by the locking device, thereby completing the tenon-mortise joint and forming the whole. Each scaffold component is in the shape of a cuboid or a polygonal body. The transverse sections of the dovetail tenons and the dovetail mortises are shaped like dovetails, arcs or steps.

The present invention provides a composition with protective effect on gastric mucosal damage, which, in parts by weight, is composed of the following components: 50-70 parts of Hericium erinaceus extract, 40-60 parts of Morchella extract, Rhizoma Rhizoma extract Compared with the prior art, the present invention is based on the theory of traditional Chinese medicine, aiming at the pathogenesis of qi deficiency in the spleen and stomach, and qi deficiency in the spleen and stomach. If the blood is not smooth, the Hericium erinaceus extract, Morchella mushroom extract, Baiji extract, and Bergamot extract are creatively used to form a traditional Chinese medicine composition. King medicine. Morchella and stomach digestion, resolve phlegm and regulate qi; Baiji absorbs astringent and generates muscle, disperses stasis and removes blood stasis, the two digest food and eliminate accumulation, promote muscle and remove rot, regulate qi and dissipate stagnation, they are ministerial medicines together, increase the blood flow of gastric mucosa, Promote oxygen supply, enhance resistance, and smooth the blood flow of the gastric mucosa, so that the body's blood is comfortable. Bergamot is an adjuvant, which has the functions of promoting qi, nourishing the liver, warming the stomach, strengthening the spleen, and relieving vomiting. Sasuke's work.

The invention relates to a foaming agent for a sound absorption foam concrete product and a preparation method for the foaming agent. The foaming agent comprises the following components in percentage by mass: 5 to 10 percent of fatty alcohol-polyoxyethylene ether sodium sulfate, 1 to 5 percent of lauryl sodium sulfate, 1 to 5 percent of sodium dodecyl benzene sulfonate, 0.1 to 2 percent of tea saponin, 1 to 5 percent of disodium ethylene diamine tetraacetate, 1 to 5 percent of modified nanometer bentonite and the balance of water. The foaming agent special for sound absorption foam concrete is stable in performance, high in foaming times and high in workability with concrete base materials, and has an adjustable pore structure.

The invention discloses a mouse sperm in vitro culture kit and a mouse fertilization method for improving the blastocyst rate. Currently, the blastocyst rate of mouse in vitro fertilization is low, which is not conducive to the development of assisted reproductive technology research based on mouse embryos. The mouse sperm in vitro culture kit for improving blastocyst rate of the present invention comprises a milliliter of mHTF, b milligram of BSA, c microgram of ERO1L recombinant protein and d microgram of DPEP2 recombinant protein. a:b:c:d=2:20:1:2. The ERO1L recombinant protein is an endoplasmic reticulum oxidoreductase 1-like protein, and its gene ID in the DNA sequence database is 50527. The DPEP2 recombinant protein is dipeptidase-2, and its gene ID in the DNA sequence database is 319446. In the present invention, ERO1L recombinant protein and DPEP2 recombinant protein are added in the kit, so that the present invention can significantly improve sperm motility and blastocyst rate.

The invention relates to a foaming agent used in ultra-low density anti-seepage foamed concrete, and a preparation method thereof. The foaming agent comprises components of, by mass: 8 to 18 parts of sodium polyoxyethylene fatty alcohol sulfate, 0.1 to 7 parts of sodium dodecylsulfate, 0.5 to 7 parts of sodium dodecylbenzene sulfonate, 4 to 15 parts of pure acrylic emulsion or styrene-acrylate emulsion, 3 to 6 parts of fatty alcohol polyoxyethylene ether, 0.5 to 2 parts of hydroxyethyl cellulose or hydroxy propyl methylcellulose, 0.1 to 5 parts of modified silicon resin or modified nano-gradebentonite, 0.1 to 0.5 parts of a thickener, and balance of water. The anti-seepage foamed concrete foaming agent provided by the invention has stable property, high foaming multiple, and good workability with a substrate. The agent can be used in a foamed concrete product with a density of 100 to 600kg / m<3>. The raw materials are easy to obtain, and the cost is low. The foaming agent can be stored for a long term with out deteriorating. With the agent, foamed concrete properties such as strength and softening coefficient can be improved.