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Digital microfluidic chip system and method for determination of heavy metal ions in water

A technology of heavy metal ions and digital microfluidics, which is applied to laboratory containers, chemical instruments and methods, and material analysis through observation of the impact on chemical indicators, can solve the problem of high testing costs, inability to meet information requirements, cumbersome operation and other issues, to achieve the effect of reducing test cost and time-consuming, facilitating integration and miniaturization, and precise droplet manipulation

Active Publication Date: 2021-12-21
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, heavy metal detection can use high-precision atomic absorption spectroscopy and atomic fluorescence spectroscopy, but the instruments used in these methods are often bulky, low-throughput, expensive, cumbersome to operate, and require more professional technicians
For spectrophotometry with relatively low instrument prices, its pretreatment is mainly based on wet chemical chromogenic reaction, and masking agents need to be added to mask interfering ions. The operation is cumbersome, and the process often takes up 90% of the entire analysis time.
At the same time, it needs to consume a lot of chemical reagents, the test cost is high, and it is not conducive to environmental protection
Although the newly developed heavy metal detection system based on continuous microfluidic chips has been improved in terms of miniaturization, automation, integration and portability, it requires mechanical components such as pumps, valves and pipelines during its operation, and there are many incidental hardware. Complex system, poor fault tolerance and stability
[0004] The above situations are far from meeting the information requirements of a laboratory in the computer age, let alone adapting to the long-term and continuous monitoring process in the harsh on-site analysis environment

Method used

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  • Digital microfluidic chip system and method for determination of heavy metal ions in water
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  • Digital microfluidic chip system and method for determination of heavy metal ions in water

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Effect test

Embodiment 1

[0032] This embodiment provides a digital microfluidic chip system for the determination of heavy metal ions in water, the system includes a digital microfluidic chip for the determination of heavy metals in water and a digital microfluidic chip peripheral device for the determination of heavy metals in water ;

[0033] The digital microfluidic chip includes a planar structure, which is divided into a work area, a pin area and an I / O interface, wherein the work area includes a droplet functional area and a detection area; the pin area is provided with a connection chip, The pins of the droplet driving circuit and the power supply; the I / O interface is connected with the sensing circuit for measuring the position of the droplet.

[0034] The digital microfluidic chip is composed of upper and lower layers of square chip structure, the upper layer is ITO conductive glass, and the lower layer is a digital control layer, which sequentially includes a base glass layer, an electrode ...

Embodiment 2

[0050] This embodiment adopts the digital microfluidic chip system of Embodiment 1, the configuration is as follows figure 1 with figure 2 As shown, configure copper sulfate standard solution 1000mL, concentration is 0.10mg / L, 50mL is used for digital microfluidic chip analysis method, 950mL is used for spectrophotometry. Cu 2+ Digital microfluidic chip analysis process such as image 3 shown, including the following steps:

[0051] (1) Cu produced from sample cell S1 2+ droplet;

[0052] (2) A deionized water droplet is generated from the A liquid storage tank to fuse with the sample droplet, and the circular motion accelerates the mixing, and the generated droplet is divided into two, one droplet is discharged into the waste liquid area, and the other enters the next step ;

[0053] (3) A masking agent (sodium malonate / sodium fluoride) droplet is generated from the B reservoir, which merges with the previous droplet, and the circular motion accelerates the reaction. T...

Embodiment 3

[0057] This embodiment adopts the digital microfluidic chip system of Embodiment 1, the configuration is as follows figure 1 with figure 2 As shown, configure nickel sulfate Ni 2+ Standard solution 1000mL, concentration 0.10mg / L, 50mL for digital microfluidic chip analysis, 950mL for spectrophotometry. Ni 2+ Digital microfluidic chip analysis process such as image 3 shown, including the following steps:

[0058] (1) Ni produced from sample cell S1 2+ droplet;

[0059] (2) A sulfuric acid droplet is generated from the A liquid storage tank to fuse with the sample droplet, and the circular movement is accelerated to mix, and the generated droplet is divided into two, one droplet is discharged into the waste liquid area, and the other enters the next step;

[0060] (3) A masking agent (sodium thiosulfate) droplet is generated from the B reservoir, which merges with the previous droplet, and the circular motion accelerates the reaction, and the generated droplet is divided...

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Abstract

The invention relates to the field of digital microfluidic chip technology and environmental monitoring, in particular to a digital microfluidic chip system and method for measuring heavy metal ions in water, the system includes a digital microfluidic chip for measuring heavy metal ions in water and a Digital microfluidic chip peripheral equipment for water heavy metal determination; the digital microfluidic chip includes a planar structure, which is divided into a work area, a pin area and an I / O interface, wherein the work area includes a droplet functional area and a detection area; The foot area is provided with pins connecting the chip, the droplet drive circuit and the power supply; the I / O interface is connected with the sensor circuit for measuring the position of the droplet. The digital microfluidic chip system reduces equipment cost, greatly saves chemical reagents, significantly improves reaction speed, and reduces test cost and time-consuming. The process does not require mechanical components such as pumps, valves and pipelines, the system is more stable, and the droplet control is more precise and reliable.

Description

technical field [0001] The invention relates to the field of digital microfluidic chip technology and environmental monitoring, in particular to a digital microfluidic chip system and method for measuring heavy metal ions in water. Background technique [0002] With the development of industry and agriculture, more and more heavy metals such as mercury, cadmium, chromium, lead, copper, zinc, nickel, barium, vanadium, etc. Concentration into the biological chain, posing a serious threat to the entire ecological environment. In order to achieve the sustainable utilization of water resources and the unification of the three major benefits of society, economy and ecology, heavy metal detection research has become the most important content in water environment research projects. [0003] At present, high-precision atomic absorption spectroscopy and atomic fluorescence spectroscopy can be used for heavy metal detection, but the instruments used in these methods are often bulky, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/78B01L3/00
CPCG01N21/64G01N21/78B01L3/5027B01L2200/10B01L2300/0861B01L2300/0887
Inventor 郑国侠王云华张倩杨玉锁
Owner DALIAN UNIV
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