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A detection method and concentration technology, which is applied in the detection field of suspended yeast concentration, can solve problems such as insufficient uniformity of distribution, large deviation of yeast concentration detection results, difficulty in achieving uniformity of yeast solution, etc., to ensure accuracy, good The effect of uniformity
Active Publication Date: 2021-12-10
广州南沙珠江啤酒有限公司
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In addition, some yeast varieties have strong cohesion, and it is difficult for the yeast solution to achieve better uniformity during mechanical shaking operation, and the uniformity of distribution in the solution is not enough
In this way, counting the yeast in the small detection volume of the counting plate will easily lead to large deviations in the detection results of the yeast concentration
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Embodiment 1
[0025] A method for detecting the concentration of suspended yeast, comprising the following steps:
[0026] 1. Sample preparation.
[0027] Take a predetermined volume of the sample liquid to be tested, add an adjusting agent, the adjusting agent contains sugar that can be decomposed and utilized by yeast, mix evenly, adjust to a predetermined volume, and obtain the liquid to be tested. details as follows:
[0028] 1. Shake the sample solution well until it is a uniform solution by visual inspection.
[0029] 2. Add 25ml of the sample solution to be tested into the dry 50ml colorimetric tube.
[0030] Pour the sample solution into the colorimetric tube, and when it is about to reach the 25ml mark, add dropwise with a rubber dropper until the lowest point of the concave of the liquid surface is flush with the 25ml mark (observe with both eyes).
[0031] 3. Add an appropriate amount of sugar solution and dilute to 50ml with pure water.
[0032] Take 5-12ml of compound syrup...
Embodiment 2
[0044] A method for detecting the concentration of suspended yeast is basically the same as the method in Example 1, the only difference being:
[0045] The selected adjusting agent is compound syrup, and the concentration of sugar in the liquid to be tested is 5%.
Embodiment 3
[0047] A method for detecting the concentration of suspended yeast is basically the same as the method in Example 1, the only difference being:
[0048] The selected adjusting agent is fructose syrup, and the concentration of sugar in the liquid to be tested is 20%.
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Abstract
The invention discloses a method for detecting the concentration of suspended yeast and relates to the technical field of beer brewing technology. The detection method comprises the following steps: sample preparation: take a predetermined volume of the sample liquid to be tested, add an adjusting agent, the adjusting agent contains sugar that can be decomposed and utilized by yeast, mix evenly, adjust to a predetermined volume, and obtain the liquid to be tested; count : Take the above-mentioned solution to be tested, inject it into a hemocytometer, and count under a microscope. The method adds wort, compound syrup, high fructose syrup, etc. to the sample solution (yeast solution) containing sugars that can be decomposed and utilized by yeast, so that the available sugar content of the yeast solution can be increased, and the yeast can be promoted in the solution. It is more dispersed and has better uniformity, which is beneficial to the subsequent counting under the microscope to ensure the accuracy of the counting results.
Description
technical field [0001] The invention relates to the technical field of beer brewing technology, in particular to a method for detecting the concentration of suspended yeast. Background technique [0002] At each stage of the beer brewing process, it is necessary to detect the concentration of suspended yeast in the solution. The most widely used detection method is the hemocytometer method. In the method, the treated yeast solution is injected into a space with a certain volume formed by the cover glass and the hemocytometer, and the yeast concentration of the sample solution is calculated by counting the yeast in the volume. [0003] However, when the hemocytometer method is used to detect the concentration of suspended yeast, the volume formed by the hemocytometer and the cover glass to count the yeast is 0.02mm 3 , very small in size. In addition, some yeast varieties have strong cohesion, so it is difficult for the yeast solution to achieve better uniformity during me...
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