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Detecting method for suspension yeast concentration

A detection method and concentration technology, applied in the field of suspension yeast concentration detection, can solve the problems of insufficient distribution uniformity, large deviation of yeast concentration detection results, and difficulty in achieving uniformity of yeast solution, so as to ensure accuracy, good quality, etc. The effect of uniformity

Active Publication Date: 2019-01-11
广州南沙珠江啤酒有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some yeast varieties have strong cohesion, and it is difficult for the yeast solution to achieve better uniformity during mechanical shaking operation, and the uniformity of distribution in the solution is not enough
In this way, counting the yeast in the small detection volume of the counting plate will easily lead to large deviations in the detection results of the yeast concentration

Method used

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  • Detecting method for suspension yeast concentration
  • Detecting method for suspension yeast concentration
  • Detecting method for suspension yeast concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for detecting the concentration of suspended yeast, comprising the following steps:

[0026] 1. Sample preparation.

[0027] Take a predetermined volume of the sample liquid to be tested, add an adjusting agent, the adjusting agent contains sugar that can be decomposed and utilized by yeast, mix evenly, adjust to a predetermined volume, and obtain the liquid to be tested. details as follows:

[0028] 1. Shake the sample solution well until it is a uniform solution by visual observation.

[0029] 2. Add 25ml of the sample solution to be tested into the dry 50ml colorimetric tube.

[0030] Pour the sample solution into the colorimetric tube, and when it is about to reach the 25ml mark, add dropwise with a rubber dropper until the lowest point of the concave of the liquid surface is flush with the 25ml mark (observe with both eyes).

[0031] 3. Add an appropriate amount of sugar solution and dilute to 50ml with pure water.

[0032] Take 5-12ml of compound syru...

Embodiment 2

[0044] A method for detecting the concentration of suspended yeast is basically the same as the method in Example 1, the only difference being:

[0045] The selected adjusting agent is compound syrup, and the concentration of sugar in the liquid to be tested is 5%.

Embodiment 3

[0047] A method for detecting the concentration of suspended yeast is basically the same as the method in Example 1, the only difference being:

[0048] The selected adjusting agent is fructose syrup, and the concentration of sugar in the liquid to be tested is 20%.

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Abstract

The invention discloses a detecting method for suspension yeast concentration and relates to the technical field of beer brewing technology. The detecting method comprises the following steps: preparing a sample: taking a preset volume of to-be-detected sample liquor, adding a regulator which contains sugars capable of being decomposed and utilized by yeast, uniformly mixing and regulating to thepreset volume, thereby acquiring the to-be-detected liquor; counting: taking the to-be-detected liquor, injecting into a blood counting chamber and counting microscopically. According to the method, sugars, such as wheat juice, compound syrup and high fructose corn syrup, capable of being decomposed and utilized by yeast are added into the to-be-detected sample liquor (yeast solution), so that thecontent of sugars capable of being utilized by yeast solution is increased, the dispersion of yeast in solution is boosted, the dispersion is high in uniformity, the subsequent microscopic counting is benefited and the accuracy of counting result is guaranteed.

Description

technical field [0001] The invention relates to the technical field of beer brewing technology, in particular to a method for detecting the concentration of suspended yeast. Background technique [0002] At each stage of the beer brewing process, it is necessary to detect the concentration of suspended yeast in the solution. The most widely used detection method is the hemocytometer method. In the method, the treated yeast solution is injected into a space with a certain volume formed by the cover glass and the hemocytometer, and the yeast concentration of the sample solution is calculated by counting the yeast in the volume. [0003] However, when the hemocytometer method is used to detect the concentration of suspended yeast, the volume formed by the hemocytometer and the cover glass to count the yeast is 0.02mm 3 , very small in size. In addition, some yeast varieties have strong cohesion, so it is difficult for the yeast solution to achieve better uniformity during me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N15/06G01N15/10
CPCG01N1/38G01N15/06G01N15/10G01N2015/1486G01N2015/1022G01N15/075
Inventor 李晓聪叶梅黄盖中
Owner 广州南沙珠江啤酒有限公司
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