Kit for determining glycated albumin

A technique of glycosylated albumin and a kit, which is applied in the field of glycosylated albumin detection kits, can solve problems such as inaccurate reflection of glycosylated hemoglobin, and achieve the effect of improving analysis sensitivity and improving analysis sensitivity

Active Publication Date: 2019-01-15
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for better results when analyzing biological samples such as blood or urine due to its ability to detect certain substances that are associated with specific diseases like cancer. It also enhance the quality control process during manufacturing processes while maintaining high levels of precision and reliability over time.

Problems solved by technology

Technics: Current methods for detecting biomarker metabolites include enzymology techniques like histochemistry analysis and Western blotting assays. However these current methods lack accuracy due to their limited range of measurement values and potential interference from various factors involved during processing steps. Therefore there remains room for improvement over existing technologies.

Method used

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  • Kit for determining glycated albumin
  • Kit for determining glycated albumin
  • Kit for determining glycated albumin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A glycosylated albumin kit with excellent accuracy recognized in the market, including reagent R1 and reagent R2, the specific components are as follows:

[0033] Reagent R1 consists of:

[0034] Tris-HCL buffer (pH8.0) 0.1mol / L

[0035] Protease 500KU / L

[0036] 5-Hydroxy-2,4,6-triiodobenzoic acid (HTIB) 10mmol / L

[0037] 6-Proclin300 3ml / L

[0038] Reagent R2 consists of:

[0039] Tris-HCL buffer (pH7.0) 0.1mol / L

[0040] Glycated amino acid oxidase 500KU / L

[0041] 4-Aminoantipyrine (4-AAP) 10mmol / L

[0042] Proclin300 3ml / L.

[0043] The test kit described in this embodiment, when in use, its assay method is to adopt automatic biochemical analyzer, such as Hitachi 7180 automatic analyzer, the operation is as follows:

[0044] Add 3 μl of normal saline, sample or calibrator, then add reagent 1 to 160 μl and incubate for 5 minutes, record the absorbance value A1 at a wavelength of 546 nm, add reagent 2 to 40 μl, record the absorbance value A2 at a wavelength o...

Embodiment 2

[0047] According to the reagent components of the present invention, a glycosylated albumin detection kit is configured, and the specific components are as follows:

[0048] Reagent R1 consists of:

[0049] Tris-HCL buffer (pH8.0) 0.1mol / L

[0050] Protease 500KU / L

[0051] 7-Hydroxy-2,4,6-triiodobenzoic acid (HTIB) 10mmol / L

[0052] 8-Proclin300 3ml / L

[0053] Reagent R2 consists of:

[0054] Tris-HCL buffer (pH7.0) 0.1mol / L

[0055] Glycated amino acid oxidase 500KU / L

[0056] 4-Aminoantipyrine (4-AAP) 10mmol / L

[0057] Proclin300 3ml / L

[0058] MgCl 2 1g / L

[0059] Glutathione 1g / L

[0060] EDTA 2g / L.

[0061] Concrete determination method is with embodiment 1.

Embodiment 3

[0063] According to the reagent components of the present invention, a glycosylated albumin detection kit is configured, and the specific components are as follows:

[0064] Tris-HCL buffer (pH8.0) 0.1mol / L

[0065] Protease 500KU / L

[0066] 9-Hydroxy-2,4,6-triiodobenzoic acid (HTIB) 10mmol / L

[0067] 10-Proclin300 3ml / L

[0068] Reagent R2 consists of:

[0069] Tris-HCL buffer (pH7.0) 0.1mol / L

[0070] Glycated amino acid oxidase 500KU / L

[0071] 4-Aminoantipyrine (4-AAP) 10mmol / L

[0072] Proclin300 3ml / L

[0073] MgCl 2 1g / L

[0074] Glutathione 2g / L

[0075] EDTA 4g / L.

[0076] Concrete determination method is with embodiment 1.

[0077] Performance experiments were performed on the kits prepared in the above examples to verify.

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Abstract

The invention discloses a kit for determining the glycated albumin, and belongs to the technical field of detection reagents in-vitro for clinical. The kit comprises a reagent R1, a reagent R2, and acalibrator. According to the kit for determining the glycated albumin, the analytical sensitivity of the kit is effectively improved, by adding the enzyme activator, which is consist of MgCl<2>, glutathione, and EDTA, to the reagent R2; and the reagent is high in accuracy and good in stability, so that the kit is beneficial to further popularization in the market.

Description

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Claims

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Application Information

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Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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