Detection method of phage

Abstract

The invention discloses a detection method of phage. The detection method comprises the following steps: S100, preparation of a culture medium: preparing a host culture medium which is suitable for growth of a phage host bacterium; S200, coating a bacterium suspension: coating a phage host bacterium suspension on the host culture medium to form a detection culture medium; S300, detection of the phage: detection of phage in gas: firstly, exposing and placing the detection culture medium in a to-be-detected region, then, culturing, and calculating the quantity of phages by using an OmeiLianski formula; and detection of the phage in a liquid: firstly, coating the detection culture medium with a to be detected liquid, then, culturing and calculating the quantity of the phages in the liquid. The detection method disclosed by the invention not only can be used for accurately detecting the quantity of the phages in gas of an industrial factory district, but also can be used for accurately detecting the quantity of the phages in liquids of the industrial factory district such as sewers and decontamination pools.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting phages. Background technique [0002] Bacteriophage was independently discovered by Twort and D.Herelle in 1907 and 1909 respectively. Bacteriophage is a general term for viruses that infect microorganisms such as bacteria, fungi, actinomycetes or spirochetes. Some of them can cause the lysis of host bacteria. Hence the name bacteriophage. As a kind of virus, bacteriophage has some characteristics unique to viruses: the individual is small; it does not have a complete cell structure; it only contains a single nucleic acid. The phage genome contains many genes, but all known phages use bacterial ribosomes, various factors required for protein synthesis, various amino acids and energy production systems in bacterial cells to achieve their own growth and proliferation , once outside the host cell, the phage can neither grow nor replicate. [0003] The fermentat...

AI Technical Summary

Problems solved by technology

[0005] 1. There is a lack of effective detection methods for detecting bacteriophages in liquids such as sewers and decontamination tanks in fermentation production industrial plants

[0006] 2. The operation of the double-layer plate agar method in the laboratory is difficult, and its accuracy is difficult to grasp. It needs professional personnel to complete it. It is not suitable for large-scale and rapid detection in the fermentation industry.

[0007] 3. It is time-consuming and labor-intensive to detect bacteriophage with a bacterioplankton sampler, and a special bacteriophage sampler is required. The price is high, which is not conducive to reducing production costs, especially affecting the development of small and medium-sized enterprises.

Images