The invention discloses a glass surface defect enhancement detection device which comprises a bright field illumination light path, a dark field illumination light path, a scanning imaging lens and a linear array image sensor. Glass to be detected is conveyed to a movement platform through a feeding conveying belt, a bright field line source, the scanning imaging lens and the linear array image sensor are opened in sequence for bright field imaging; then the bright field line source is closed, a laser emits laser light, the laser light is converted into a line source through a Powell prism and a collimator, and dark field imaging is carried out by the scanning imaging lens and the linear array image sensor; statistic analysis is carried out on surface defect information of the glass by an intelligent system according to data obtained after bright field imaging and dark field imaging and the results are summarized and then output; through judgment on severity of defects, the effect of detecting out unqualified products is improved and the product quality is improved.