Somatic embryogenesis and plant regeneration culturing method for Koelreuteria paniculata
A culture method and embryogenesis technology are applied in the cultivation of plant somatic embryogenesis and plant regeneration, and in the field of somatic embryogenesis and plant regeneration of Luan tree. The effect of increasing the number of regenerated plants and the number of somatic embryos
Active Publication Date: 2019-01-18
BEIJING FORESTRY UNIVERSITY
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2) There is physiological isolation
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Embodiment 1
[0038] The cultivation of embodiment 1 eucalyptus somatic embryogenesis and plant regeneration
[0039] 1. Materials and methods
[0040] 1.1 Experimental materials
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The invention discloses a somatic embryogenesis and plant regeneration culturing method for Koelreuteria paniculata. The method comprises the following steps: (1) culturing a Koelreuteria paniculata seed as an explant to obtain a Koelreuteria paniculata aseptic seedling; (2) inoculating a callus inducing medium with leaves and stem segments of the Koelreuteria paniculata aseptic seedling, and performing culturing to obtain an embryogenic callus; (3) performing somatic embryo differentiation culturing on the embryogenic callus in a somatic embryo development medium to obtain a Koelreuteria paniculata somatic cotyledon embryo; (4) performing cotyledon embryo maturation culturing on the Koelreuteria paniculata somatic cotyledon embryo in a maturation medium; (5) performing germination culturing on a mature cotyledon embryo in a seed germination medium to obtain a complete plant; (6) inoculating a rooting medium with stem segments of the complete plant for rooting culturing. The culturingmethod is high in Koelreuteria paniculata somatic embryogenesis rate, a large number of somatic embryos are produced, the number of regenerated Koelreuteria paniculata plants is increased, and a foundation is provided for asexual reproduction culturing, molecular assisted selection and the like of the Koelreuteria paniculata.
Description
technical field [0001] The invention relates to a cultivation method for plant somatic embryogenesis and plant regeneration, in particular to a cultivation method for eucalyptus somatic embryogenesis and plant regeneration, and belongs to the field of cultivation of eucalyptus somatic embryogenesis and plant regeneration. Background technique [0002] The eucalyptus is the most important representative plant in the eucalyptus genus, and it plays an extremely important role in landscaping applications. There are four species of eucalyptus plants in taxonomy, three of which are native tree species unique to China, namely eucalyptus (Koelreuteria paniculata Laxm.), Taiwan eucalyptus (K.formosana Hayata.) .). Among them, there is also a variety of K. integrifoliola Merr. in the compound feather eucalyptus. In addition, there is a class of eucalyptus, Fiji eucalyptus, distributed in the Fiji Islands. Compared with other eucalyptus plants, eucalyptus does not have complete bipi...
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CPCA01H4/001A01H4/008
Inventor 安新民杨雄高凯郭婷杨晓宇赵田芸
Owner BEIJING FORESTRY UNIVERSITY
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