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Culture method for somatic embryogenesis and plant regeneration of Eucalyptus eucalyptus

A culture method and technology of somatic embryos, which are applied in the field of cultivation of somatic embryos and plant regeneration of eucalyptus, can solve the problems of physiological isolation, etc., achieve high incidence of somatic embryos, increase the number of regenerated plants, and increase the number of somatic embryos.

Active Publication Date: 2022-01-04
BEIJING FORESTRY UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

2) There is physiological isolation

Method used

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  • Culture method for somatic embryogenesis and plant regeneration of Eucalyptus eucalyptus
  • Culture method for somatic embryogenesis and plant regeneration of Eucalyptus eucalyptus
  • Culture method for somatic embryogenesis and plant regeneration of Eucalyptus eucalyptus

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Embodiment 1

[0038] The cultivation of embodiment 1 eucalyptus somatic embryogenesis and plant regeneration

[0039] 1. Materials and methods

[0040] 1.1 Experimental materials

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Abstract

The invention discloses a cultivation method for eucalyptus somatic embryogenesis and plant regeneration; the method comprises: (1) cultivating eucalyptus seeds as explants to obtain eucalyptus aseptic seedlings; (2) cultivating eucalyptus aseptic seedlings; Leaves and stems were inoculated into callus induction medium and cultured to obtain embryogenic callus; (3) The embryogenic callus was cultured for differentiation of somatic embryos in somatic embryo development medium to obtain eucalyptus somatic cotyledon embryos (4) Carry out the maturation and development of the cotyledon embryo of the eucalyptus somatic cotyledon embryo in the mature development medium; (5) carry out the germination culture of the mature cotyledon embryo in the seed germination medium to obtain a complete plant; (6) complete Fragments of plant stems are inoculated into rooting medium for rooting culture. The culture method of the invention has a high occurrence rate of eucalyptus somatic embryos, produces a large number of somatic embryos, and increases the number of regenerated plants of eucalyptus eucalyptus.

Description

technical field [0001] The invention relates to a cultivation method for plant somatic embryogenesis and plant regeneration, in particular to a cultivation method for eucalyptus somatic embryogenesis and plant regeneration, and belongs to the field of cultivation of eucalyptus somatic embryogenesis and plant regeneration. Background technique [0002] The eucalyptus is the most important representative plant in the eucalyptus genus, and it plays an extremely important role in landscaping applications. There are four species of eucalyptus plants in taxonomy, three of which are native tree species unique to China, namely eucalyptus (Koelreuteria paniculata Laxm.), Taiwan eucalyptus (K.formosana Hayata.) .). Among them, there is also a variety of K. integrifoliola Merr. in the compound feather eucalyptus. In addition, there is a class of eucalyptus, Fiji eucalyptus, distributed in the Fiji Islands. Compared with other eucalyptus plants, eucalyptus does not have complete bipi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 安新民杨雄高凯郭婷杨晓宇赵田芸
Owner BEIJING FORESTRY UNIVERSITY
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