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A kind of embryo culture solution and a kind of treatment method of somatic cell clone embryo

A technology for embryo culture medium and somatic cell cloning, which is applied in the field of in vitro cell cloning and can solve the problems of reducing embryo reprogramming efficiency and low cloning efficiency.

Active Publication Date: 2022-03-11
WENS FOODSTUFF GRP CO LTD
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Problems solved by technology

Histone deacetylase, on the other hand, can deacetylate histones, inhibit transcription, and reduce the efficiency of embryonic reprogramming.
Use fully differentiated somatic cells to construct cloned embryos. Somatic cells are oriented to transcription, and their histone deacetylase content is high, which inhibits transcriptional activation and gene expression, resulting in low cloning efficiency. Therefore, inhibition of histone deacetylation is used. Lyase is an effective means to improve cloning efficiency

Method used

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  • A kind of embryo culture solution and a kind of treatment method of somatic cell clone embryo
  • A kind of embryo culture solution and a kind of treatment method of somatic cell clone embryo
  • A kind of embryo culture solution and a kind of treatment method of somatic cell clone embryo

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Embodiment Construction

[0023] Taking pig nuclear transfer embryo construction as an example below, the present invention will be further described in detail in conjunction with the accompanying drawings.

[0024] 1. Isolation and culture of donor cells

[0025] The donor cells come from the super boar ear tissue of Guangdong Wenshi Group. The pig ear skin is cut and stored in DMEM culture medium at 4°C and transported back to the laboratory. After the pig ear skin tissue pieces are cut into pieces, the tissue fragments are washed with DMEM Resuspend with appropriate amount of fetal bovine serum and transfer to culture dish, 37°C, 5% CO 2 , cultured in a saturated humidity environment. After 5-7 hours, add DMEM culture medium containing 10% fetal bovine serum, and subculture when the cells grow to 90% confluence, and use the somatic cells passed to the second generation as donor cells for nuclear transfer.

[0026] 2. Oocyte collection and maturation culture

[0027] The ovaries obtained from sows...

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Abstract

The invention discloses an embryo culture solution, which contains 200-400nM of the compound represented by the structural formula (1). The embryo culture solution has obvious effects, and the blastocyst rate of cloned embryos is significantly increased, thereby improving the efficiency of cloning technology. The invention also discloses a treatment method for somatic cell cloned embryos. The treatment method for somatic cell cloned embryos is simple in operation and obvious in effect, and can significantly improve the cloning process including but not limited to pigs, cattle, sheep, mice, and humans. Embryo Efficiency in Vitro Development of Somatic Cell Nuclear Transfer Embryos.

Description

technical field [0001] The invention relates to the technical field of cell in vitro cloning, in particular to an embryo culture solution and a treatment method for somatic cell cloned embryos. Background technique [0002] Although many animals have obtained adult individuals through somatic cell cloning technology, the low efficiency of this technology is still the main reason for limiting its application. Current research shows that the low efficiency is mainly caused by abnormal reprogramming of cloned embryos, such as histone hypoacetylation and DNA hypermethylation. Most of the reprogramming of the nuclei of cloned embryo donors occurs in early embryos before the embryonic genome is activated, and histone acetylation is an epigenetic modification that is one of the key factors for gene activation in embryos. After histone acetyltransferase acts on histone to acetylate it, it will lead to a loose chromatin conformation, allowing the access of transcription factors, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/14C12N2500/12C12N2500/30C12N2501/998
Inventor 吴珍芳石俊松周荣罗绿花麦然标余婉娴贺晓燕蔡更元
Owner WENS FOODSTUFF GRP CO LTD
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