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Determination method for HPLC-ELSD fingerprint spectrum of Lotrimin Sol Lotrimin

A technology of Huangshi Xiangsheng Pills and its determination method, which is applied to the determination of fingerprints of Huangshi Xiangsheng Pills preparations, and in the field of fingerprints of Huangshi Xiangsheng Pills preparations, can solve problems such as evaluation, and achieve the effect of perfect quality control technology

Active Publication Date: 2019-01-18
WUXI JIYU SHANHE PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection method for the quality evaluation of Huangshi Xiangsheng Pills in the Pharmacopoeia is to use high-performance liquid phase-ELSD detector to measure the content of peiminin A and peiminin B, and there is no overall evaluation of the preparation. Therefore, Huang’s Xiangsheng Pills HPLC-ELSD fingerprint method to evaluate the drug as a whole

Method used

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  • Determination method for HPLC-ELSD fingerprint spectrum of Lotrimin Sol Lotrimin
  • Determination method for HPLC-ELSD fingerprint spectrum of Lotrimin Sol Lotrimin
  • Determination method for HPLC-ELSD fingerprint spectrum of Lotrimin Sol Lotrimin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Step (1) Preparation of the test solution: take Huangshi Xiangsheng pills, pulverize them, take about 4g, accurately weigh them, put them in a conical flask, add 50ml of 85% methanol, ultrasonicate for 1 hour, let cool, filter, and evaporate to dryness , dissolved in 25ml of water, transferred to a separatory funnel, extracted 3 times with ethyl acetate, 25ml each time, combined the ethyl acetate extracts, evaporated to dryness, dissolved the residue with methanol, transferred to a 5ml volumetric flask, and diluted with methanol to scale, shake well.

[0103] Step (2) The concentrations of the reference substance solutions were prepared as follows: the concentration of the forsythin reference substance was 0.3125 mg / ml, the concentration of the chrysophanol reference substance was 0.4981 mg / ml, and the concentration of the rhein reference substance was 0.5306 mg / ml.

[0104] Step (3) need testing solution, reference substance solution is injected into chromatograph, obt...

Embodiment 2

[0114] For the fingerprint determination of batch 170801 of Huangshi Xiangsheng Pills, the specific steps are as follows:

[0115] Step (1) Preparation of the test solution: take Huangshi Xiangsheng pills, pulverize them, take about 4g, accurately weigh them, put them in a conical flask, add 50ml of 85% methanol, ultrasonicate for 1 hour, let cool, filter, and evaporate to dryness , dissolved in 25ml of water, transferred to a separatory funnel, extracted 3 times with ethyl acetate, 25ml each time, combined the ethyl acetate extracts, evaporated to dryness, dissolved the residue with methanol, transferred to a 5ml volumetric flask, and diluted with methanol to scale, shake well.

[0116] Step (2) The concentrations of the reference substance solutions were prepared as follows: the concentration of the forsythin reference substance was 0.3125 mg / ml, the concentration of the chrysophanol reference substance was 0.4981 mg / ml, and the concentration of the rhein reference substance...

Embodiment 3

[0127] For the fingerprint determination of batch 171003 of Huangshi Xiangsheng Pills, the specific steps are as follows:

[0128] Step (1) Preparation of the test solution: take Huangshi Xiangsheng pills, pulverize them, take about 4g, accurately weigh them, put them in a conical flask, add 50ml of 85% methanol, ultrasonicate for 1 hour, let cool, filter, and evaporate to dryness , dissolved in 25ml of water, transferred to a separatory funnel, extracted 3 times with ethyl acetate, 25ml each time, combined the ethyl acetate extracts, evaporated to dryness, dissolved the residue with methanol, transferred to a 5ml volumetric flask, and diluted with methanol to scale, shake well.

[0129] Step (2) The concentrations of the reference substance solutions were prepared as follows: the concentration of the forsythin reference substance was 0.3125 mg / ml, the concentration of the chrysophanol reference substance was 0.4981 mg / ml, and the concentration of the rhein reference substance...

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Abstract

The invention relates to a determination method for the HPLC-ELSD fingerprint spectrum of Lotrimin Sol Lotrimin. The determination method comprises the following steps of: preparing a sample solution;preparing a control sample solution; injecting the solutions into a liquid chromatograph (evaporative light scattering detector) to determine; and judging whether the quality data of the Lotrimin SolLotrimin obtained by the determination method is qualified or not on the basis of the standard fingerprint spectrum. The method is simple, rapid, accurate, stable and reliable, can be used for the quality control of a Lotrimin Sol Lotrimin sample, and can comprehensively, objectively and scientifically evaluate the quality of the Lotrimin Sol Lotrimin so as to guarantee the effectiveness of the preparation.

Description

technical field [0001] The invention relates to a method for analyzing the fingerprints of traditional Chinese medicines, in particular to a method for determining the fingerprints of Huangshi Xiangsheng Pill preparations, and the obtained fingerprints of Huangshi Xiangsheng Pills preparations. technical background [0002] The composition of traditional Chinese medicine and compound preparations in my country is very complicated, because its therapeutic effect is carried out through overall coordination. Therefore, it should be taken as a whole when evaluating its quality. However, the general quality standard is to evaluate the content of a certain component in a traditional Chinese medicine or a compound prescription. It is difficult to control its intrinsic quality like this. Therefore, in order to improve and highlight the overall characteristics of traditional Chinese medicine, establish quality control standards and evaluation models that conform to the characterist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8686G01N30/88G01N2030/8809
Inventor 甘国峰李璐於江华
Owner WUXI JIYU SHANHE PHARM CO LTD
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