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Uses of Rosa tomentosa Fruit Extract

A technology of extracts and roses, which is applied in the directions of medical preparations, applications, and drug combinations containing active ingredients to achieve the effect of inhibiting deposition and high safety.

Active Publication Date: 2021-07-16
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are no relevant researches on the prevention and treatment of non-alcoholic fatty liver and liver cell oxidative damage by Rosa tomentosa fruit extract at home and abroad.

Method used

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  • Uses of Rosa tomentosa Fruit Extract
  • Uses of Rosa tomentosa Fruit Extract
  • Uses of Rosa tomentosa Fruit Extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of various phenolic components of Rosa tomentosa fruit: Freeze-dry and pulverize Rosa tomophy fruit fruit, extract Rosa tomentosa fruit powder ultrasonically with 80% methanol solution, the solid-liquid ratio g:mL is 1:5, Ultrasonic extraction at 40°C for 30min, filtration, repeated extraction of the filter residue twice, combined filtrate; low pressure, 40°C rotary evaporation concentrated to obtain sample 1; the concentrated solution was passed through ion exchange resin (Ameberlite XAD-7), with a mass concentration of 1% Wash the column with formic acid aqueous solution to remove non-polyphenolic substances (such as sugar, protein, etc.), then elute with methanol to obtain polyphenolic substances, collect the methanol eluate, spin evaporate, concentrate, and freeze-dry to obtain the crude polyphenols of Rosa tomentosa fruit. The extract, weighed, and stored at -20°C, is sample 2; take sample 2, dissolve it in pure water, and put it on Sephadex LH-20, and t...

Embodiment 2

[0021] Determination of the total phenol content in the phenolic components of Rosa tomentosa fruit: Dissolve the freeze-dried sample with 80% methanol solution to make a 1mg / mL sample solution, take 1mL and add it to a 10mL centrifuge tube, then add 6mL distilled water and 0.5mL folinol, after mixing, add 1.5mL mass concentration 20% Na 2 CO 3 Finally, distill the entire reaction system to 10 mL with distilled water; return to room temperature after a 70°C water bath (10 min), and measure the absorbance at 765 nm with a microplate reader. Using gallic acid as the equivalent to make a standard curve, the total phenolic content of the sample is expressed as: mg gallic acid / g dry weight of the extract, and the results are shown in Table 1;

[0022] Content of total flavonoids in each material component of Rosa tomentosa fruit: dissolve the freeze-dried extract with 70% ethanol, take 1mL sample solution in a centrifuge tube, add 2.5 mL 70% ethanol, and then add 0.15 mL 5% NaNO ...

Embodiment 3

[0026] Embodiment 3: Using UHPLC-ESI-MS to analyze the compound composition in the fruit of Rosa tomentosa, the specific operations are as follows:

[0027]UHPLC-ESI-HRMS / MS was used to conduct qualitative and quantitative analysis of phenolic substances in the phenolic components of Rosa tomentosa fruit; the chromatographic conditions of the liquid phase were Reprospher 100 C18 analytical column (1.8 μm, 100 × 2 mm), The mobile phase was 0.1% formic acid water (A) and mass spectrometry grade acetonitrile (B), the flow rate was 0.3 mL / min, the injection volume was 3 µL, and the column temperature was set at 35 °C. The more suitable mobile phase gradient is: 0.01 min, 5% (B); 3 min, 11% (B); 12 min, 13% (B); 14 min, 18.5% (B); 20 min, 18.5% ( B); 26 min, 35% (B); 26.5 min, 52% (B); 28 min, 52% (B); the main mass spectrometer condition is full scan (150~1000 m / z); ESI ion source temperature The temperature was 320°C; the ionization voltage was 3.2kV; the auxiliary air flow was ...

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Abstract

The invention discloses the use of the fruit extract of Rosa tortoise, namely the application of the fruit extract of Rosa tortoise, in the preparation of medicines and health food for preventing and treating non-alcoholic fatty liver and liver damage; The functions of the extracts to prevent and treat non-alcoholic fatty liver and hepatocyte damage were verified respectively; the experimental results confirmed that each extract component can effectively inhibit the deposition of fat in liver cells, and has a good function of preventing and treating non-alcoholic fatty liver; At the same time, each component can significantly inhibit H 2 O 2 Induced ROS generation in hepatocytes, protecting hepatocytes from H 2 O 2 The oxidative damage of Rosa chinensis fruit is low, widely distributed, simple to grow, and easy to obtain raw materials; in a word, the fruit extract of Rosa chinensis has a good development in the preparation of drugs and health food for the prevention and treatment of non-alcoholic fatty liver and liver damage. Take advantage of prospects.

Description

technical field [0001] The invention relates to the application of Rosa tomentosa fruit extract in the preparation of medicines for preventing and treating non-alcoholic fatty liver and liver oxidative damage, and belongs to the technical field of food or medicine. Background technique [0002] With the development of social economy, people's material life is becoming more and more abundant, and at the same time, the incidence of non-alcoholic fatty liver is also increasing; it is generally believed that the cause of non-alcoholic fatty liver is mainly "secondary strike". The first blow is that a high-fat diet leads to a large amount of fat deposition in liver cells; the second blow is that when excessive fat deposition in liver cells increases the oxidative stress state of liver cells, it is more likely to cause mitochondrial dysfunction and Oxidative damage to liver cells, eventually leading to non-alcoholic steatohepatitis. [0003] There are many clinical symptoms of n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/738A61P1/16A23L33/00A61K131/00
CPCA23V2002/00A61K36/738A61K2236/15A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23L33/00A61P1/16A23V2200/32
Inventor 蔡圣宝高凡丁庞明杰王春丽张缘月杨俊娴
Owner KUNMING UNIV OF SCI & TECH
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