Application of prinsepia utilis royle pericarp and pulp extract
A technology of green thorn fruit and extract, which is applied in the field of food or medicine to achieve the effect of high safety, considerable market prospect and broad market prospect
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Embodiment 1
[0015] fresh green thorn fruit ( P. utilis Royle ) after removing the seeds, homogenate the pulp and peel; the crushed homogenate (50g) is mixed with formic acid-methanol (containing 1% formic acid and 80% methanol), the ratio of solid to liquid g:mL is 1:5, Ultrasonic extraction in a water bath for 30 minutes, ultrasonic power 210W, ultrasonic temperature 35°C, filtered to obtain the extract, and the filter residue was extracted twice, combined with the extract, and then concentrated under reduced pressure at 40°C to remove the solvent; the concentrate was placed on an Amberlite XAD-7HP column, The column was washed with 1% aqueous formic acid to remove non-polyphenolic substances, and the sample was eluted with pure methanol. The methanol eluate was collected, concentrated, and freeze-dried to obtain the total phenolic component, sample 1. Take a part of the total phenolic component and dissolve the sample with deionized water of pH 7, put it on a SephadexLH-20 column, wash...
Embodiment 2
[0017] Determination of the total phenolic content in each component sample of the green thorn fruit: dissolve the freeze-dried sample with a mass concentration of 80% methanol solution to make a 1mg / mL sample solution, take 1 mL and add it to a 10 mL centrifuge tube, then add 6 mL of distilled water and 0.5mL folinol, mix well and add 1.5mL 20% Na 2 CO 3 solution, and finally dilute the entire reaction system to 10 mL with distilled water; return to room temperature after 70 °C water bath (10 min), and measure the absorbance at 765 nm with a microplate reader. Using gallic acid as the equivalent to make a standard curve, the total phenolic content of the sample is expressed as: mg gallic acid / g dry weight of the extract, and the results are shown in Table 1;
[0018] Content of total flavonoids in the samples of each component of P. japonica: dissolve the freeze-dried extract with 70% ethanol, take 1 mL of the sample solution in a centrifuge tube, add 2.5 mL of 70% ethanol, ...
Embodiment 3
[0023] UHPLC-ESI-MS was used to analyze the composition of the compounds in each component of the green thorn fruit, and the specific operation was as follows: the chromatographic conditions of the liquid phase were Reprospher 100 C18 analytical column (100 × 2 mm, 1.8 μm), and the mobile phase was 2.0% formic acid Water (A) and mass spectrometry grade acetonitrile (B), a suitable mobile phase gradient is: 0.01–5 min, 10% B; 5–8 min, 10%–15% B, 8–15 min, 15%–25 % B, 15–20 min, 25%–10% B, 20–23 min, 10%B. The flow rate was 0.2 mL / min, the injection volume was 2.0 µL, and the column temperature was set at 35 °C. The mass spectrometry conditions were: full scan (150-1000 m / z); capillary temperature 320 °C; ionization voltage 3.3 kV; auxiliary gas flow rate 8 L / min; sheath gas flow rate 32 L / min; 350°C. Total phenols and flavonoids were detected in negative ion mode, anthocyanins were detected in positive ion mode, and the detection results of main phenolic substances are shown ...
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