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Application of prinsepia utilis royle pericarp and pulp extract

A technology of green thorn fruit and extract, which is applied in the field of food or medicine to achieve the effect of high safety, considerable market prospect and broad market prospect

Inactive Publication Date: 2019-02-22
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic and foreign research reports on the peel and pulp of the green thorn fruit, especially the research on the extracts of the green thorn fruit peel and pulp as an α-glucosidase inhibitor to reduce postprandial blood sugar has not been reported in the literature

Method used

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  • Application of prinsepia utilis royle pericarp and pulp extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] fresh green thorn fruit ( P. utilis Royle ) after removing the seeds, homogenate the pulp and peel; the crushed homogenate (50g) is mixed with formic acid-methanol (containing 1% formic acid and 80% methanol), the ratio of solid to liquid g:mL is 1:5, Ultrasonic extraction in a water bath for 30 minutes, ultrasonic power 210W, ultrasonic temperature 35°C, filtered to obtain the extract, and the filter residue was extracted twice, combined with the extract, and then concentrated under reduced pressure at 40°C to remove the solvent; the concentrate was placed on an Amberlite XAD-7HP column, The column was washed with 1% aqueous formic acid to remove non-polyphenolic substances, and the sample was eluted with pure methanol. The methanol eluate was collected, concentrated, and freeze-dried to obtain the total phenolic component, sample 1. Take a part of the total phenolic component and dissolve the sample with deionized water of pH 7, put it on a SephadexLH-20 column, wash...

Embodiment 2

[0017] Determination of the total phenolic content in each component sample of the green thorn fruit: dissolve the freeze-dried sample with a mass concentration of 80% methanol solution to make a 1mg / mL sample solution, take 1 mL and add it to a 10 mL centrifuge tube, then add 6 mL of distilled water and 0.5mL folinol, mix well and add 1.5mL 20% Na 2 CO 3 solution, and finally dilute the entire reaction system to 10 mL with distilled water; return to room temperature after 70 °C water bath (10 min), and measure the absorbance at 765 nm with a microplate reader. Using gallic acid as the equivalent to make a standard curve, the total phenolic content of the sample is expressed as: mg gallic acid / g dry weight of the extract, and the results are shown in Table 1;

[0018] Content of total flavonoids in the samples of each component of P. japonica: dissolve the freeze-dried extract with 70% ethanol, take 1 mL of the sample solution in a centrifuge tube, add 2.5 mL of 70% ethanol, ...

Embodiment 3

[0023] UHPLC-ESI-MS was used to analyze the composition of the compounds in each component of the green thorn fruit, and the specific operation was as follows: the chromatographic conditions of the liquid phase were Reprospher 100 C18 analytical column (100 × 2 mm, 1.8 μm), and the mobile phase was 2.0% formic acid Water (A) and mass spectrometry grade acetonitrile (B), a suitable mobile phase gradient is: 0.01–5 min, 10% B; 5–8 min, 10%–15% B, 8–15 min, 15%–25 % B, 15–20 min, 25%–10% B, 20–23 min, 10%B. The flow rate was 0.2 mL / min, the injection volume was 2.0 µL, and the column temperature was set at 35 °C. The mass spectrometry conditions were: full scan (150-1000 m / z); capillary temperature 320 °C; ionization voltage 3.3 kV; auxiliary gas flow rate 8 L / min; sheath gas flow rate 32 L / min; 350°C. Total phenols and flavonoids were detected in negative ion mode, anthocyanins were detected in positive ion mode, and the detection results of main phenolic substances are shown ...

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Abstract

The invention discloses an application of prinsepia utilis royle pericarp and pulp extract as an alpha-glycosidase inhibitor and belongs to the technical field of food or medicines. The application comprises the following steps: by taking prinsepia utilis royle pericarp and pulp as a raw material; extracting extract of the prinsepia utilis royle pericarp and pulp by means of an organic reagent ora mixed solution of water and the organic reagent; and then carrying out purification and separation through macroporous resin, a gel and a C18 small column to obtain different extract of the prinsepia utilis royle pericarp and pulp. An in vitro alpha-glycosidase activity experiment shows that all extract can inhibit activity of alpha-glycosidase effectively so as to inhibit hydrolysis of carbohydrates such as starch in the gastrointestinal tract to reduce absorption of glucose. Therefore, the extract can serve as the alpha-glycosidase inhibitor for preparing health-care food and a drug of postprandial blood sugar.

Description

technical field [0001] The invention relates to the application of the extract of the green thorn fruit as an alpha-glucosidase inhibitor, and belongs to the technical field of food or medicine. Background technique [0002] With the continuous improvement of people's material life, unhealthy high-sugar diet and bad living habits induce the occurrence of various chronic diseases. Among them, diabetes has seriously threatened people's health, brought heavy pressure to the medical system all over the world, and seriously reduced people's quality of life. Lowering and controlling postprandial blood sugar is an important way to prevent and treat diabetes, and thus has been widely valued by people. Carbohydrates need to be hydrolyzed by the corresponding digestive enzymes in the gastrointestinal tract before they can be absorbed by the body. These carbohydrates are mainly hydrolyzed by α-glucosidase. Therefore, by inhibiting the activity of α-glucosidase, reducing and delaying ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P3/10A23L19/00A23L33/105A61K131/00
CPCA61P3/10A23L19/09A23L33/105A61K36/73A23V2002/00A23V2200/328A23V2250/21A23V2300/14
Inventor 蔡圣宝张璇王春丽庞明杰郑越马爽曾内辉
Owner KUNMING UNIV OF SCI & TECH
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